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Journal of Molecular Neuroscience

, Volume 56, Issue 4, pp 808–814 | Cite as

Neuroprotective Mechanisms of 9-Hydroxy Epinootkatol Against Glutamate-Induced Neuronal Apoptosis in Primary Neuron Culture

  • Hong Zhao
  • Zhi-Hong Ji
  • Chao Liu
  • Xin-Yu YuEmail author
Article

Abstract

Glutamate-induced neuronal apoptosis has been implicated in the pathogenesis of neurological disorders. 9-Hydroxy epinootkatol (9OHEN), a sesquiterpene compound with neuroprotective activity against glutamate-induced neuronal apoptosis, was isolated from Alpinia oxyphylla Miquel in our previous study. In this study, we investigated the neuroprotective mechanisms of 9OHEN against glutamate-induced neuronal apoptosis in primary cultured neurons. The results from this study demonstrated that 9OHEN protected cortical neurons from glutamate-induced neuronal apoptosis via inhibiting glutamate-induced activation of caspase-3, inhibiting glutamate-induced reactive oxygen species (ROS) production, inhibiting glutamate-induced nitric oxide (NO) production, and downregulating glutamate-induced neuronal nitric oxide synthase (nNOS) expression. This study suggest that 9OHEN might have therapeutic potential in treating glutamate-mediated neurological diseases.

Keywords

9-Hydroxy epinootkatol Neuroprotection Glutamate Excitotoxicity Apoptosis 

Notes

Acknowledgments

This study was supported by the grant from the National Natural Science Foundation of China (grant number: 30472073) and the grant from the Education Bureau of Liaoning Provence (Grant No. L2011042).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Laboratory of Neuroscience, College of MedicineDalian UniversityDalianPeople’s Republic of China
  2. 2.Department of Traditional Chinese Medicine, College of MedicineDalian UniversityDalianPeople’s Republic of China

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