Journal of Molecular Neuroscience

, Volume 55, Issue 4, pp 845–853 | Cite as

Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells into Motorneuron-like Cells on Electrospun Gelatin Membrane

  • Faezeh Faghihi
  • Esmaeil Mirzaei
  • Arash Sarveazad
  • Jafar Ai
  • Somayeh Ebrahimi Barough
  • Abolfazl Lotfi
  • Mohammad Taghi JoghataeiEmail author


Human bone marrow-derived mesenchymal stem cells are potent types of cells with self renewal ability and immunomodulatory properties. They not only have the capacity to differentiate into mesodermal lineages, but they are also capable to transdifferentiate into neural cells in vitro and in vivo. From a biological point of view, the specification of cell fate in the central nervous system is largely dictated by retinoic acid and sonic hedgehog. In addition with inductive molecules, electrospun three dimensional (3D) scaffolds with similar properties to natural extracellular matrix represent a physiological environment that could better resemble the in vivo microenvironment in comparison with two dimensional culture systems. In this regard, the aim of this study was to examine whether induction of human BM-MSCs with retinoic acid (RA) and sonic hedgehog (Shh) in combination with electrospun gelatin scaffold could lead to better differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) into motorneuron-like cells in vitro.


Human bone marrow mesenchymal stem cells Motorneuron Electrospun gelatin membrane 



This study was financially supported by Iran National Science Foundation (INSF).

Conflicts of Interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Faezeh Faghihi
    • 1
  • Esmaeil Mirzaei
    • 2
  • Arash Sarveazad
    • 3
  • Jafar Ai
    • 4
    • 5
  • Somayeh Ebrahimi Barough
    • 4
  • Abolfazl Lotfi
    • 6
  • Mohammad Taghi Joghataei
    • 1
    • 3
    • 7
    Email author
  1. 1.Cellular and Molecular Research CenterIran University of Medical SciencesTehranIran
  2. 2.Department of Medical Nanotechnology, School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  3. 3.Department of Anatomy, School of MedicineIran University of Medical SciencesTehranIran
  4. 4.Department of Tissue Engineering, School of Advanced Technologies in MedicineTehran University of Medical SciencesTehranIran
  5. 5.Brain and Spinal Injury Research Center, Imam Khomeini HospitalTehran University of Medical SciencesTehranIran
  6. 6.Department of BiotechnologyNational Institute of Genetic Engineering and BiotechnologyTehranIran
  7. 7.Department of Neuroscience, School of Advanced Technologies in MedicineIran University of Medical SciencesTehranIran

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