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Journal of Molecular Neuroscience

, Volume 54, Issue 3, pp 331–341 | Cite as

Structural and Morphometric Comparison of the Molar Teeth in Pre-eruptive Developmental Stage of PACAP-Deficient and Wild-Type Mice

  • B. Sandor
  • K. Fintor
  • Sz. Felszeghy
  • T. Juhasz
  • D. Reglodi
  • L. Mark
  • P. Kiss
  • A. Jungling
  • B. D. Fulop
  • A. D. Nagy
  • H. Hashimoto
  • R. Zakany
  • A. Nagy
  • A. TamasEmail author
Article

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic neuropeptide with widespread distribution. It plays pivotal role in neuronal development. PACAP-immunoreactive fibers have been found in the tooth pulp, and recently, it has been shown that PACAP may also play a role in the regeneration of the periodontium after luxation injuries. However, there is no data about the effect of endogenous PACAP on tooth development. Ectodermal organogenesis including tooth development is regulated by different members of bone morphogenetic protein (BMP), fibroblast growth factor (FGF), hedgehog (HH), and Wnt families. There is also a growing evidence to support the hypothesis that PACAP interacts with sonic hedgehog (SHH) receptor (PTCH1) and its downstream target (Gli1) suggesting its role in tooth development. Therefore, our aim was to study molar tooth development in mice lacking endogenous PACAP. In this study morphometric, immunohistochemical and structural comparison of molar teeth in pre-eruptive developmental stage was performed on histological sections of 7-day-old wild-type and PACAP-deficient mice. Further structural analysis was carried out with Raman microscope. The morphometric comparison of the 7-day-old samples revealed that the dentin was significantly thinner in the molars of PACAP-deficient mice compared to wild-type animals. Raman spectra of the enamel in wild-type mice demonstrated higher diversity in secondary structure of enamel proteins. In the dentin of PACAP-deficient mice higher intracrystalline disordering in the hydroxyapatite molecular structure was found. We also obtained altered SHH, PTCH1 and Gli1 expression level in secretory ameloblasts of PACAP-deficient mice compared to wild-type littermates suggesting that PACAP might play an important role in molar tooth development and matrix mineralization involving influence on SHH signaling cascade.

Keywords

PACAP Deficient Raman Tooth Development SHH PTCH1 Gli1 

Notes

Acknowledgements

We are grateful to Tamas Papp and Tunde Palne Terdik for technical assistance. This work was supported by PTE-MTA “Lendulet” Program, Arimura Foundation, OTKA K104984, PD109644, TAMOP 4.2.2.A-11/1/KONV-2012-0024, TAMOP 4.2.4.A/2-11-1-2012-0001 “National Excellence Program”, Bolyai Scholarship, Grants from University of Debrecen (RH/885/2013), Grants-in-Aid for Scientific Research, KAKENHI, Grant Numbers 26293020 and 26670122.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • B. Sandor
    • 1
    • 2
  • K. Fintor
    • 3
  • Sz. Felszeghy
    • 4
    • 5
  • T. Juhasz
    • 4
  • D. Reglodi
    • 2
  • L. Mark
    • 6
  • P. Kiss
    • 2
  • A. Jungling
    • 2
  • B. D. Fulop
    • 2
  • A. D. Nagy
    • 2
  • H. Hashimoto
    • 7
  • R. Zakany
    • 4
  • A. Nagy
    • 1
  • A. Tamas
    • 2
    Email author
  1. 1.Department of Dentistry, Oral and Maxillofacial Surgery, Medical SchoolUniversity of PecsPecsHungary
  2. 2.Department of Anatomy, PTE-MTA “Lendulet” PACAP Research Team, Medical SchoolUniversity of PecsPecsHungary
  3. 3.Department of Mineralogy Geochemistry and Petrology, Faculty of Science and InformaticsUniversity of SzegedSzegedHungary
  4. 4.Department of Anatomy, Histology and Embryology, Faculty of MedicineUniversity of DebrecenDebrecenHungary
  5. 5.Faculty of DentistryUniversity of DebrecenDebrecenHungary
  6. 6.Department of Analytical Biochemistry, Institute of Biochemistry and Medical Chemistry, Imaging Center for Life and Material Sciences, Janos Szentagothai Research Center, Medical SchoolUniversity of PecsPecsHungary
  7. 7.Graduate School of Pharmaceutical SciencesOsaka UniversityOsakaJapan

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