Journal of Molecular Neuroscience

, Volume 55, Issue 1, pp 91–98 | Cite as

Glial-Like Differentiation Potential of Human Mature Adipocytes

  • Antonella Poloni
  • Giulia Maurizi
  • Federica Foia
  • Eleonora Mondini
  • Domenico Mattiucci
  • Patrizia Ambrogini
  • Davide Lattanzi
  • Stefania Mancini
  • Massimo Falconi
  • Saverio Cinti
  • Attilio Olivieri
  • Pietro Leoni
Article
First Online:
Received:
Accepted:

Abstract

The potential ability to differentiate dedifferentiated adipocytes into a neural lineage is attracting strong interest as an emerging method of producing model cells for the treatment of a variety of neurological diseases. Here, we describe the efficient conversion of dedifferentiated adipocytes into a neural-like cell population. These cells grew in neurosphere-like structures and expressed a high level of the early neuroectodermal marker Nestin. These neurospheres could proliferate and express stemness genes, suggesting that these cells could be committed to the neural lineage. After neural induction, NeuroD1, Sox1, Double Cortin, and Eno2 were not expressed. Patch clamp data did not reveal different electrophysiological properties, indicating the inability of these cells to differentiate into mature neurons. In contrast, the differentiated cells expressed a high level of CLDN11, as demonstrated using molecular method, and stained positively for the glial cell markers CLDN11 and GFAP, as demonstrated using immunocytochemistry. These data were confirmed by quantitative results for glial cell line-derived neurotrophic factor production, which showed a higher secretion level in neurospheres and the differentiated cells compared with the untreated cells. In conclusion, our data demonstrate morphological, molecular, and immunocytochemical evidence of initial neural differentiation of mature adipocytes, committing to a glial lineage.

Keywords

Dedifferentiated adipocytes Neurosphere Differentiation Glial-like cells 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Antonella Poloni
    • 1
  • Giulia Maurizi
    • 1
  • Federica Foia
    • 1
  • Eleonora Mondini
    • 2
  • Domenico Mattiucci
    • 1
  • Patrizia Ambrogini
    • 3
  • Davide Lattanzi
    • 3
  • Stefania Mancini
    • 1
  • Massimo Falconi
    • 4
  • Saverio Cinti
    • 2
  • Attilio Olivieri
    • 1
  • Pietro Leoni
    • 1
  1. 1.Clinica di Ematologia, Dipartimento di Scienze Cliniche e MolecolariUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Dipartimento di Medicina Sperimentale e ClinicaUniversità Politecnica delle MarcheAnconaItaly
  3. 3.Dipartimento di Scienze della Terra, della Vita e dell’Ambiente, Sezione di FisiologiaUniversità di Urbino Carlo BoUrbinoItaly
  4. 4.Clinica Chirurgia del PancreasUniversità Politecnica delle Marche, Ospedali RiunitiAnconaItaly

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