Journal of Molecular Neuroscience

, Volume 53, Issue 2, pp 251–261 | Cite as

Hyperbaric Oxygen Treatment Produces an Antinociceptive Response Phase and Inhibits Astrocyte Activation and Inflammatory Response in a Rat Model of Neuropathic Pain

  • Bai-Song Zhao
  • Ling-Xin Meng
  • Yuan-Yuan Ding
  • Yan-Yan Cao
Article

Abstract

Hyperbaric oxygen (HBO) treatment has been proven to be a promising candidate for protection of the nervous system after acute injury in animal models of neuropathic pain. The purposes of this study were to examine the antinociceptive response phase induced by HBO treatment in a model of neuropathic pain and to determine the dependence of the treatment's mechanism of alleviating neuropathic pain on the inhibition of spinal astrocyte activation. Neuropathic pain was induced in rats by chronic constriction injury of the sciatic nerve. Mechanical threshold and thermal latency were tested preoperatively and for 1 week postoperatively, four times daily at fixed time points. Methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) parameters were used as indices of oxidative stress response and tested before and after the treatment. The inflammatory cytokines interleukin (IL)-1β and IL-10 were assayed in the sciatic nerve were with enzyme-linked immunoassay. Glial fibrillary acidic protein activation in the spinal cord was evaluated immunohistochemically. The rats exhibited temporary allodynia immediately after HBO treatment completion. This transient allodynia was closely associated with changes in MDA and SOD levels. A single HBO treatment caused a short-acting antinociceptive response phase. Repetitive HBO treatment led to a long-acting antinociceptive response phase and inhibited astrocyte activation. These results indicated that HBO treatment played a dual role in the aggravation and alleviation of neuropathic pain, though the aggravated pain effect (transient allodynia) was far less pronounced than the antinociceptive phase. Astrocyte inhibition and anti-inflammation may contribute to the antinociceptive effect of HBO treatment after nerve injury.

Keywords

Hyperbaric oxygen Neuropathic pain Antinociception Astrocyte Chronic constriction injury 

Abbreviations

HBO

Hyperbaric oxygen

TNF

Tumor necrosis factor

CCI

Chronic constriction injury

ATA

Atmospheres absolute pressure

MWT

Mechanical withdrawal threshold

TWL

Thermal withdrawal latency

AB

Avidin-biotin

GFAP

Glial fibrillary acidic protein

ROS

Oxygen species

CREB

cAMP response element-binding protein

MDA

Methane dicarboxylic aldehyde

SOD

Superoxide dismutase

Notes

Acknowledgments

This work was supported by the Science and Technology Plan Project and B. Braun Anesthesia Scientific Research Fund (grant no. F10-205-1-41 and BBF 2012-012). We thank Dr. Cui Jian-Jun for supporting this study.

Conflict of Interest

There are no ethical/legal conflicts involved in the article.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Bai-Song Zhao
    • 1
  • Ling-Xin Meng
    • 1
  • Yuan-Yuan Ding
    • 2
  • Yan-Yan Cao
    • 1
  1. 1.Department of AnesthesiologyChina Medical University, Shengjing HospitalShenyangChina
  2. 2.Department of PainChina Medical University, Shengjing HospitalShenyangChina

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