Journal of Molecular Neuroscience

, Volume 52, Issue 1, pp 148–155 | Cite as

MicroRNA-124 (miR-124) Regulates Ku70 Expression and is Correlated with Neuronal Death Induced by Ischemia/Reperfusion

  • Fei Zhu
  • Jing-Li Liu
  • Jing-Pin Li
  • Fang Xiao
  • Zhao-Xia Zhang
  • Lei Zhang
Article

Abstract

MicroRNAs are small, non-coding RNA molecules that regulate gene expression, and miR-124 is the most abundant miRNA in the brain. Studies have shown that miR-124 is clearly reduced in the ischemic brain after stroke; however, the role of miR-124 after stroke is less well studied. Using TargetScan, MicroCosm Targets version 5, and microRNA.org databases, we identified miR-124 as a possible regulator of the DNA repair protein Ku70. We validated that Ku70 is a target for miR-124 with a luciferase reporter activity assay. Moreover, adult rats subjected to focal cerebral ischemia exhibited a substantial reduction of miR-124 expression, which was inversely upregulated by Ku70 expression. In vivo treatment with miR-124 antagomir effectively enhanced Ku70 mRNA and protein levels in the ischemic region. Furthermore, knockdown of cerebral miR-124 reduced cell death and infarct size and improved neurological outcomes. Our data demonstrate that miR-124 is an endogenous regulator of Ku70 that improves ischemia/reperfusion (I/R)-induced brain injury and dysfunction.

Keywords

Cerebral ischemia Ku70 MicroRNA Apoptosis 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Fei Zhu
    • 1
  • Jing-Li Liu
    • 1
  • Jing-Pin Li
    • 1
  • Fang Xiao
    • 1
  • Zhao-Xia Zhang
    • 1
  • Lei Zhang
    • 1
  1. 1.Department of Neurology, The First Affiliated HospitalGuangxi Medical UniversityNanningChina

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