Journal of Molecular Neuroscience

, Volume 51, Issue 3, pp 779–787 | Cite as

Ceftriaxone Treatment Affects the Levels of GLT1 and ENT1 As Well As Ethanol Intake in Alcohol-Preferring Rats

  • Youssef SariEmail author
  • Sai N. Sreemantula
  • Moonnoh R. Lee
  • Doo-Sup Choi


Studies have demonstrated that deletion of equilibrative nucleoside transporter 1 (ENT1) is associated with reduced glutamate transporter 1 (GLT1) level, and consequently increased ethanol intake. In this study, we measured changes in GLT1 and ENT1 levels in prefrontal cortex (PFC), and nucleus accumbens (NAc) core and shell associated with alcohol drinking in alcohol-preferring (P) rats. We examined, then, whether ceftriaxone (CEF) would affect both GLT1 and ENT1 levels in these brain regions. P rats were given 24-h concurrent access to 15 and 30 % ethanol, water, and food for 5 weeks. On Week 6, P rats received 100 mg/kg CEF (i.p.) or a saline vehicle for five consecutive days. Ethanol intake was measured daily for 8 days starting on the first day of injections. We found a significant reduction in daily ethanol intake in CEF-treated group, starting on Day 2 of injections. Western blot for GLT1 and binding assay for ENT1 revealed downregulation of GLT1 level, whereas ENT1 levels were increased in the NAc core and NAc shell, respectively, but not in the PFC in saline vehicle group. Importantly, CEF treatment reversed these effects in both NAc core and shell. These findings provide evidence for potential regulatory effects of CEF on both GLT1 and ENT1 expression in reducing ethanol intake.


ENT1 GLT1 EAAT2 Alcohol dependence Glutamate 



This work was supported by Award Number R01AA019458 (Y.S.) and R01AA018779 (D-S. C.) from the National Institutes on Alcohol Abuse and Alcoholism. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute on Alcohol Abuse and Alcoholism or the National Institutes of Health. The authors would like to thank Mrs. Charisse Montgomery for editing this manuscript.

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Youssef Sari
    • 1
    Email author
  • Sai N. Sreemantula
    • 1
  • Moonnoh R. Lee
    • 2
  • Doo-Sup Choi
    • 2
  1. 1.Department of Pharmacology, College of Pharmacy and Pharmaceutical SciencesUniversity of ToledoToledoUSA
  2. 2.Department of Molecular Pharmacology and Experimental TherapeuticsMayo Clinic College of MedicineRochesterUSA

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