Journal of Molecular Neuroscience

, Volume 48, Issue 3, pp 638–646 | Cite as

Expression of VIP and its Receptors in the Testis of the Spotted Ray Torpedo marmorata (Risso 1880)

  • Marisa Agnese
  • Luigi Rosati
  • Francesco Muriano
  • Salvatore Valiante
  • Vincenza Laforgia
  • Piero AndreuccettiEmail author
  • Marina Prisco


The aim of this work was to study, by immunoprecipitation, in situ hybridization and immunohistochemistry, and the expression of the vasoactive intestinal peptide (VIP) and of its receptors (VPAC1R and VPAC2R) in the testis of a nonmammalian vertebrate, the cartilaginous fish Torpedo marmorata. We demonstrated that, differently from mammals, VIP and VPAC2R were widely distributed in the testicular cells while the VPAC1R had a limited distribution. In details, we showed that VIP and VPAC2R were present in mitotic and differentiating germ cells as well as in the cells involved in the steroidogenesis, i.e., Leydig, Sertoli cells, and prespermatogonia and spermatogonia. The possibility that VIP is involved in the spermatogenesis and particularly in the steroidogenesis of T. marmorata is discussed.


VIP VPAC1VPAC2Torpedo marmorata Testis Cartilaginous fish 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Marisa Agnese
    • 1
  • Luigi Rosati
    • 1
  • Francesco Muriano
    • 1
  • Salvatore Valiante
    • 1
  • Vincenza Laforgia
    • 1
  • Piero Andreuccetti
    • 1
    Email author
  • Marina Prisco
    • 1
  1. 1.Department of Biological SciencesUniversity of Naples Federico IINaplesItaly

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