Journal of Molecular Neuroscience

, Volume 48, Issue 1, pp 144–153 | Cite as

Electromagnetic Field Stimulation Potentiates Endogenous Myelin Repair by Recruiting Subventricular Neural Stem Cells in an Experimental Model of White Matter Demyelination

  • Mohammad Amin Sherafat
  • Motahareh Heibatollahi
  • Somayeh Mongabadi
  • Fatemeh Moradi
  • Mohammad Javan
  • Abolhassan Ahmadiani
Article

Abstract

Electromagnetic fields (EMFs) may affect the endogenous neural stem cells within the brain. The aim of this study was to assess the effects of EMFs on the process of toxin-induced demyelination and subsequent remyelination. Demyelination was induced using local injection of lysophosphatidylcholine within the corpus callosum of adult female Sprague–Dawley rats. EMFs (60 Hz; 0.7 mT) were applied for 2 h twice a day for 7, 14, or 28 days postlesion. BrdU labeling and immunostaining against nestin, myelin basic protein (MBP), and BrdU were used for assessing the amount of neural stem cells within the tissue, remyelination patterns, and tracing of proliferating cells, respectively. EMFs significantly reduced the extent of demyelinated area and increased the level of MBP staining within the lesion area on days 14 and 28 postlesion. EMFs also increased the number of BrdU- and nestin-positive cells within the area between SVZ and lesion as observed on days 7 and 14 postlesion. It seems that EMF potentiates proliferation and migration of neural stem cells and enhances the repair of myelin in the context of demyelinating conditions.

Keywords

Electromagnetic field stimulation Myelin repair Multiple sclerosis Lysophosphatidylcholine Endogenous neural stem cells Rat 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mohammad Amin Sherafat
    • 1
  • Motahareh Heibatollahi
    • 1
  • Somayeh Mongabadi
    • 1
  • Fatemeh Moradi
    • 1
  • Mohammad Javan
    • 2
  • Abolhassan Ahmadiani
    • 1
  1. 1.Neuroscience Research CenterShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Department of Physiology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran

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