Journal of Molecular Neuroscience

, Volume 48, Issue 2, pp 360–367

Regulation by L-Type Calcium Channels of Endocytosis: An Overview

  • Juliana M. Rosa
  • Carmen Nanclares
  • Angela Orozco
  • Inés Colmena
  • Ricardo de Pascual
  • Antonio G. García
  • Luis Gandía
Article

DOI: 10.1007/s12031-012-9786-5

Cite this article as:
Rosa, J.M., Nanclares, C., Orozco, A. et al. J Mol Neurosci (2012) 48: 360. doi:10.1007/s12031-012-9786-5

Abstract

The exocytotic neurotransmitter release process is tightly coupled to the membrane retrieval (endocytosis) process since both are calcium-dependent processes. For instance, at the adrenal chromaffin cells, catecholamine release is regulated by Ca2+ entry through L, N and PQ subtypes of voltage-dependent calcium channels (VDCC). The contribution of a given VDCC subtype to exocytosis may differ according to the animal species studied, with L channels contributing only about 20 % to the total Ca2+ entry in bovine chromaffin cells. However, data from electrophysiological experiments with membrane capacitance measurements and fluorescence imaging with FM dyes indicate that Ca2+ entry through the L-type channels seems to selectively regulate the endocytotic response after the application of a single depolarizing pulse to voltage-clamped bovine chromaffin cells. How do L-type channels control endocytosis remains to be fully clarified. By using specific antibodies against VDCC subtypes and endocytic proteins (i.e. dynamin and clathrin), it has been demonstrated that VDCC subtypes do not co-localise with these proteins. On the other hand, electrophysiological data suggest that the particular mode of sustained Ca2+ entry through slow-inactivating L-type channels could be responsible for the activation of the endocytotic machinery. Here, we present an overview of the current understanding of the contribution of L-type channels during endocytosis.

Keywords

Calcium channels Exocytosis Fast endocytosis Chromaffin cells 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Juliana M. Rosa
    • 1
    • 4
  • Carmen Nanclares
    • 1
    • 2
  • Angela Orozco
    • 1
    • 2
  • Inés Colmena
    • 1
    • 2
  • Ricardo de Pascual
    • 1
    • 2
  • Antonio G. García
    • 1
    • 2
    • 3
  • Luis Gandía
    • 1
    • 2
  1. 1.Instituto Teófilo Hernando, Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain
  2. 2.Departamento de Farmacología y Terapéutica, Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain
  3. 3.Servicio de Farmacología Clínica, Instituto de Investigación Sanitaria, Hospital Universitario de la Princesa, Facultad de MedicinaUniversidad Autónoma de MadridMadridSpain
  4. 4.MRC Laboratory of Molecular BiologyCambridgeUK

Personalised recommendations