Journal of Molecular Neuroscience

, Volume 41, Issue 1, pp 210–218

Global microRNA Expression Profiling of Caenorhabditis elegans Parkinson's Disease Models

  • Suvi Asikainen
  • Martina Rudgalvyte
  • Liisa Heikkinen
  • Kristiina Louhiranta
  • Merja Lakso
  • Garry Wong
  • Richard Nass
Article

Abstract

MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs that may be involved in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify potential gene expression changes in 115 annotated miRNAs in PD-associated Caenorhabditis elegans models that either overexpress human A53T α-synuclein or have mutations within the vesicular catecholamine transporter (cat-1) or parkin (pdr-1) ortholog. Here, we show that 12 specific miRNAs are differentially regulated in the animals overexpressing α-synuclein, five in cat-1, and three in the pdr-1 mutants. The family of miR-64 and miR-65 are co-underexpressed in the α-synuclein transgenic and cat-1 strains, and members of let-7 family co-underexpressed in the α-synuclein and pdr-1 strains; mdl-1 and ptc-1 genes are target candidates for miR-64 and miR-65 and are overexpressed in α-synuclein transgenic as well as miR-64/65 (tm3711) knockout animals. These results indicate that miRNAs are differentially expressed in C. elegans PD models and suggest a role for these molecules in disease pathogenesis.

Keywords

microRNA Parkinson's disease Neurodegeneration Microarray qRT-PCR Caenorhabditis elegans 

Supplementary material

12031_2009_9325_MOESM1_ESM.docx (23 kb)
Supplementary Table 1All miRNAs detected in the microarray experiment (DOCX 22 kb)
12031_2009_9325_MOESM2_ESM.docx (15 kb)
Supplementary Table 2miRNAs missing from the NCode miRNA microarray platform (DOCX 15 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Suvi Asikainen
    • 1
    • 2
  • Martina Rudgalvyte
    • 1
    • 2
  • Liisa Heikkinen
    • 1
    • 2
  • Kristiina Louhiranta
    • 1
    • 2
  • Merja Lakso
    • 1
    • 2
  • Garry Wong
    • 1
    • 2
  • Richard Nass
    • 3
  1. 1.Department of BiosciencesKuopio UniversityKuopioFinland
  2. 2.Department of Neurobiology, A.I.Virtanen Institute for Molecular SciencesKuopio UniversityKuopioFinland
  3. 3.Department of Pharmacology and Toxicology, Center for Environmental Health, Stark Neuroscience Research InstituteIndiana University School of MedicineIndianapolisUSA

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