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Journal of Molecular Neuroscience

, Volume 41, Issue 1, pp 138–144 | Cite as

Presence of Kisspeptin-like Immunoreactivity in Human Adrenal Glands and Adrenal Tumors

  • Kazuhiro Takahashi
  • Itaru Shoji
  • Akiko Shibasaki
  • Ichiro Kato
  • Keisuke Hiraishi
  • Hajime Yamamoto
  • Kiriko Kaneko
  • Osamu Murakami
  • Ryo Morimoto
  • Fumitoshi Satoh
  • Sadayoshi Ito
  • Kazuhito Totsune
Article

Abstract

Kisspeptins are neuropeptides which activate the hypothalamo-pituitary gonadal axis and are considered to play important physiological roles in the reproduction. Kisspeptins have also been reported to stimulate the aldosterone secretion from the adrenal cortex. However, the expression of kisspeptins in human adrenal glands and adrenal tumors has not been clarified yet. We, therefore, studied the presence of kisspeptin-like immunoreactivity (LI) in human adrenal glands and adrenal tumors (adrenocortical adenomas, adrenocortical carcinomas, and pheochromocytomas) by radioimmunoassay and immunocytochemistry. Kisspeptin-LI was detected in all the tissues examined; normal portions of adrenal glands (3.0 ± 2.3 pmol/g wet weight, n = 21, mean ± SD), aldosterone-producing adenomas (4.6 ± 3.3 pmol/g wet weight, n = 10), cortisol-producing adenomas (2.7 ± 1.4 pmol/g wet weight, n = 14), adrenocortical carcinomas (1.7 ± 0.2 pmol/g wet weight, n = 4), and pheochromocytomas (1.8 ± 0.8 pmol/g wet weight, n = 6). There was no significant difference in kisspeptin-LI levels among them. Immunocytochemistry showed positive kisspeptin-immunostaining in normal adrenal glands, with stronger immunostaining found in the medulla. Furthermore, positive kisspeptin-immunostaining was found in all types of adrenal tumors examined; adrenocortical adenomas, adrenocortical carcinomas, and pheochromocytomas. The intensity of kisspeptin-immunostaining in these adrenal tumors was, however, not so strong as that in normal adrenal medulla. The present study has shown for the first time the presence of kisspeptin-LI in adrenal glands and adrenal tumors.

Keywords

Kisspeptin Metastin Adrenal Tumor Adenoma Carcinoma Pheochromocytoma 

Notes

Acknowledgments

This work was supported partly by The Salt Science Research Foundation, No. 0910, and by Grant-in-aids for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

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Copyright information

© Humana Press 2009

Authors and Affiliations

  • Kazuhiro Takahashi
    • 1
  • Itaru Shoji
    • 1
  • Akiko Shibasaki
    • 1
  • Ichiro Kato
    • 1
  • Keisuke Hiraishi
    • 1
  • Hajime Yamamoto
    • 1
    • 4
  • Kiriko Kaneko
    • 1
  • Osamu Murakami
    • 2
  • Ryo Morimoto
    • 2
  • Fumitoshi Satoh
    • 2
  • Sadayoshi Ito
    • 2
  • Kazuhito Totsune
    • 3
  1. 1.Department of Endocrinology and Applied Medical ScienceTohoku University Graduate School of MedicineSendaiJapan
  2. 2.Division of Nephrology, Endocrinology, and Vascular Medicine, Department of MedicineTohoku University Graduate School of MedicineSendaiJapan
  3. 3.Department of Clinical Pharmacology and TherapeuticsTohoku University Graduate School of Pharmaceutical Sciences and MedicineSendaiJapan
  4. 4.Takeda General HospitalAizu-WakamatsuJapan

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