Journal of Molecular Neuroscience

, Volume 36, Issue 1–3, pp 299–309

Expression of Phosphorylated cAMP Response Element Binding Protein (p-CREB) in Bladder Afferent Pathways in VIP−/− Mice with Cyclophosphamide (CYP)-Induced Cystitis

  • Dorthe G. Jensen
  • Simon Studeny
  • Victor May
  • James Waschek
  • Margaret A. Vizzard
Article

Abstract

The expression of phosphorylated cAMP response element binding protein (p-CREB) in dorsal root ganglia (DRG) with and without cyclophosphamide (CYP)-induced cystitis (150 mg/kg, i.p; 48 h) was determined in VIP−/− and wild-type (WT) mice. p-CREB immunoreactivity (IR) was determined in bladder (Fast blue) afferent cells. Nerve growth factor (NGF) bladder content was determined by enzyme-linked immunosorbent assays. Basal expression of p-CREB-IR in DRG of VIP−/− mice was (p ≤ 0.01) greater in L1, L2, L5-S1 DRG compared to WT mice. CYP treatment in WT mice increased (p ≤ 0.05) p-CREB-IR in L1, L2, L5-S1 DRG. CYP treatment in VIP−/− mice (p ≤ 0.01) increased (p ≤ 0.01) p-CREB-IR in L6-S1 DRG compared to WT with CYP. In WT mice, bladder afferent cells (20–38%) in DRG expressed p-CREB-IR under basal conditions. With CYP, p-CREB-IR increased in bladder afferent cells (60–65%; L6-S1 DRG) in WT mice. In VIP−/− mice, bladder afferent cells (12–58%) expressed p-CREB-IR under basal conditions, and CYP increased p-CREB expression (78–84%) in L6-S1 DRG. Urinary bladder NGF expression in VIP−/− mice under basal conditions or after cystitis was significantly greater than WT. Detrusor smooth muscle thickness was significantly increased in VIP−/− mice. Bladder NGF expression may contribute to differences in p-CREB expression.

Keywords

Inflammation Urinary bladder Afferent neurons Growth factors Cytokines 

Copyright information

© Humana Press 2008

Authors and Affiliations

  • Dorthe G. Jensen
    • 1
  • Simon Studeny
    • 2
  • Victor May
    • 3
  • James Waschek
    • 4
  • Margaret A. Vizzard
    • 2
    • 3
  1. 1.Departments of Pharmacology and Pharmacotherapy, Faculty of Pharmaceutical SciencesUniversity of CopenhagenCopenhagenDenmark
  2. 2.Department of NeurologyUniversity of Vermont College of MedicineBurlingtonUSA
  3. 3.Department of Anatomy/NeurobiologyUniversity of Vermont College of MedicineBurlingtonUSA
  4. 4.University of CaliforniaLos AngelesUSA

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