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Journal of Molecular Neuroscience

, Volume 33, Issue 1, pp 56–66 | Cite as

Role of Liver and Plasma Lipoproteins in Selective Transport of n-3 Fatty Acids to Tissues: A Comparative Study of 14C-DHA and 3H-Oleic Acid Tracers

  • Alla Polozova
  • Norman SalemJrEmail author
Article

Abstract

We conducted a study aimed at a direct comparison of the plasma dynamics and uptake of docosahexaenoic (DHA) and oleic (OA) fatty acids by various organs. 14C-DHA and 3H-OA were intravenously co-injected into mice. At 5 min after injection, more than 40% of the 14C-DHA, but less than 20% of the 3H-OA, labels was associated with the liver. Heart uptake of 14C-DHA was three to four times greater compared to the 3H-OA label. Brain incorporation of 14C-DHA slowly rose to 0.7% at 24 h, but it remained at the 1–1.5% level for 3H-OA. Total 14C activity in plasma reached 2% of the injected dose at 20 min and leveled off at 0.5% after 1.5 h. Fifteen percent of 14C-DHA plasma activity at 30 min was associated with non-esterified fatty acids, whereas about 85% was recovered in triglycerides in very low-density lipoprotein (VLDL) and LDL fractions. Only 30% of 3H-OA derived activity was found in the VLDL fraction at 30 min. All 3H activity in plasma at later time points was in catabolite fractions. These findings demonstrate that liver plays an important role in the initial selectivity for DHA. It is likely that DHA is specifically taken up by liver, esterified, loaded into lipoproteins, and then delivered to brain, heart, and other target tissues.

Keywords

Omega-3 Docosahexaenoic acid DHA Lipoproteins Fatty acid transport 

Abbreviations

OA

oleic acid

DHA

docosahexaenoic acid

CE

cholesterol ester

NEFA

non-esterified fatty acid

PL

phospholipid

PC

phosphatidylcholine

PE

phosphatidylethanolamine

TG

triglycerides

DG

diglycerides

MG

monoglycerides

PUFA

polyunsaturated fatty acid

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  1. 1.Laboratory of Membrane Biochemistry and BiophysicsNational Institute on Alcohol Abuse and AlcoholismBethesdaUSA

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