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NanoBiotechnology

, Volume 3, Issue 2, pp 89–95 | Cite as

Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells

  • Jie Lu
  • Monty Liong
  • Sean Sherman
  • Tian Xia
  • Michael Kovochich
  • Andre E. Nel
  • Jeffrey I. ZinkEmail author
  • Fuyuhiko TamanoiEmail author
Article

Abstract

Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37°C than at 4°C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.

Keywords

mesoporous silica nanoparticle endocytosis hydrophobic drugs drug delivery cancer 

Notes

Acknowledgments

This work is supported by US NIH grants CA32737 (F.T.) and ES015498 (A.N.), NSF grant DMR0346601 (J.Z.) and a grant from Edna E. and Susan E. Riley foundation. Monty Liong and Michael Kovochich are supported by the UC Lead Campus for Nanotoxicology Training and Research, funded by UC TSR&TP.

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Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Jie Lu
    • 1
    • 4
  • Monty Liong
    • 2
    • 4
  • Sean Sherman
    • 1
    • 4
  • Tian Xia
    • 3
    • 4
  • Michael Kovochich
    • 3
    • 4
  • Andre E. Nel
    • 3
    • 4
  • Jeffrey I. Zink
    • 2
    • 4
    Email author
  • Fuyuhiko Tamanoi
    • 1
    • 4
    • 5
    Email author
  1. 1.Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology InstituteUniversity of California Los AngelesLos AngelesUSA
  2. 2.Department of Chemistry and BiochemistryUniversity of California Los AngelesLos AngelesUSA
  3. 3.Department of MedicineUniversity of California Los AngelesLos AngelesUSA
  4. 4.California NanoSystems InstituteUniversity of California Los AngelesLos AngelesUSA
  5. 5.Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, JCCCUniversity of California Los AngelesLos AngelesUSA

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