Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells
- 391 Downloads
Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37°C than at 4°C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.
Keywordsmesoporous silica nanoparticle endocytosis hydrophobic drugs drug delivery cancer
This work is supported by US NIH grants CA32737 (F.T.) and ES015498 (A.N.), NSF grant DMR0346601 (J.Z.) and a grant from Edna E. and Susan E. Riley foundation. Monty Liong and Michael Kovochich are supported by the UC Lead Campus for Nanotoxicology Training and Research, funded by UC TSR&TP.
- 3.Ebbesen M, Jensen TG. J Biomed Biotechnol 2006;2006:51516.Google Scholar
- 9.Chen J, Cha Y, Yuksel K, Gracy R, August J. J Biol Chem 1988;263:8754–8.Google Scholar
- 10.Lichstein HC, Soule MH. J Bacteriol 1944;47:231–8.Google Scholar
- 12.Wessing A, Bertram G, Zierold K. J Comp Physiol 1993;163:452.Google Scholar
- 14.Harvey WR, Maddrell SHP, Telfer WH, Wieczorek H. Amer Zool 1998;38:426–41.Google Scholar
- 15.Samson F, Donoso JA, Heller-Bettinger I, Watson D, Himes RH. J Pharmacol Exp Ther 1979;208:411–7.Google Scholar
- 18.Kim JS, Yoon TJ, Yu KN, Noh MS, Woo M, Kim BG, et al. J Vet Sci 2006;7:321–6.Google Scholar