, Volume 3, Issue 2, pp 89–95 | Cite as

Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells

  • Jie Lu
  • Monty Liong
  • Sean Sherman
  • Tian Xia
  • Michael Kovochich
  • Andre E. Nel
  • Jeffrey I. ZinkEmail author
  • Fuyuhiko TamanoiEmail author


Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37°C than at 4°C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.


mesoporous silica nanoparticle endocytosis hydrophobic drugs drug delivery cancer 



This work is supported by US NIH grants CA32737 (F.T.) and ES015498 (A.N.), NSF grant DMR0346601 (J.Z.) and a grant from Edna E. and Susan E. Riley foundation. Monty Liong and Michael Kovochich are supported by the UC Lead Campus for Nanotoxicology Training and Research, funded by UC TSR&TP.


  1. 1.
    Reynolds AR, Moein MS, Hodivala-Dilke K. Trends Mol Med 2003;9:2–4.CrossRefGoogle Scholar
  2. 2.
    Van Vlerken LE, Amiji MM. Expert Opin Drug Deliv 2006;3:205–16.CrossRefGoogle Scholar
  3. 3.
    Ebbesen M, Jensen TG. J Biomed Biotechnol 2006;2006:51516.Google Scholar
  4. 4.
    Brannon-Peppas L, Blanchette JO. Adv Drug Deliv Rev 2004;56:1649–59.CrossRefGoogle Scholar
  5. 5.
    Kresge CT, Leonowicz ME, Roth WJ, Vartuli JC, Beck JS. Nature 1992;359:710–2.CrossRefGoogle Scholar
  6. 6.
    Han YJ, Stucky GD, Butler A. J Am Chem Soc 1999;121:9897–8.CrossRefGoogle Scholar
  7. 7.
    Lu J, Liong M, Zink JI, Tamanoi F. Small 2007;3:1341–6.CrossRefGoogle Scholar
  8. 8.
    Canonico PG, Bird JWC. J Cell Biol 1969;43:367–71.CrossRefGoogle Scholar
  9. 9.
    Chen J, Cha Y, Yuksel K, Gracy R, August J. J Biol Chem 1988;263:8754–8.Google Scholar
  10. 10.
    Lichstein HC, Soule MH. J Bacteriol 1944;47:231–8.Google Scholar
  11. 11.
    Heuser JE, Anderson RG. J Cell Biol 1989;108:389–400.CrossRefGoogle Scholar
  12. 12.
    Wessing A, Bertram G, Zierold K. J Comp Physiol 1993;163:452.Google Scholar
  13. 13.
    Pearse BM, Crowther RA. Annu Rev Biophys Biophys Chem 1987;16:49–68.CrossRefGoogle Scholar
  14. 14.
    Harvey WR, Maddrell SHP, Telfer WH, Wieczorek H. Amer Zool 1998;38:426–41.Google Scholar
  15. 15.
    Samson F, Donoso JA, Heller-Bettinger I, Watson D, Himes RH. J Pharmacol Exp Ther 1979;208:411–7.Google Scholar
  16. 16.
    Roma MG, Milkiewicz P, Elias E, Coleman R. Hepatology 2000;32:1342–56.CrossRefGoogle Scholar
  17. 17.
    Chung TH, Wu SH, Yao M, Lu CW, Lin YS, Hung Y, et al. Biomaterials 2007;28:2959–66.CrossRefGoogle Scholar
  18. 18.
    Kim JS, Yoon TJ, Yu KN, Noh MS, Woo M, Kim BG, et al. J Vet Sci 2006;7:321–6.Google Scholar
  19. 19.
    Gemeinhart RA, Luo D, Saltzman WM. Biotechnol Prog 2005;21:532–7.CrossRefGoogle Scholar
  20. 20.
    Xing X, He X, Peng J, Wang K, Tan W. J Nanosci Nanotechnol 2005;5:1688–93.CrossRefGoogle Scholar
  21. 21.
    Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT. J Am Chem Soc 1971;93:2325–7.CrossRefGoogle Scholar
  22. 22.
    Slowing I, Trewyn BG, Lin VS. J Am Chem Soc 2006;128:14792–3.CrossRefGoogle Scholar
  23. 23.
    Luo D, Saltzman WM. Nat Biotechnol 2000;18:893–5.CrossRefGoogle Scholar
  24. 24.
    Jin Y, Kannan S, Wu M, Zhao JX. Chem Res Toxicol 2007;20:1126–33.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Jie Lu
    • 1
    • 4
  • Monty Liong
    • 2
    • 4
  • Sean Sherman
    • 1
    • 4
  • Tian Xia
    • 3
    • 4
  • Michael Kovochich
    • 3
    • 4
  • Andre E. Nel
    • 3
    • 4
  • Jeffrey I. Zink
    • 2
    • 4
    Email author
  • Fuyuhiko Tamanoi
    • 1
    • 4
    • 5
    Email author
  1. 1.Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology InstituteUniversity of California Los AngelesLos AngelesUSA
  2. 2.Department of Chemistry and BiochemistryUniversity of California Los AngelesLos AngelesUSA
  3. 3.Department of MedicineUniversity of California Los AngelesLos AngelesUSA
  4. 4.California NanoSystems InstituteUniversity of California Los AngelesLos AngelesUSA
  5. 5.Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, JCCCUniversity of California Los AngelesLos AngelesUSA

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