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NanoBiotechnology

, Volume 1, Issue 3, pp 277–278 | Cite as

Microsystems for the analysis of cellular signal transduction

  • Alexander Ganser
  • Günter Roth
  • Karsten Köhler
  • Thomas André
  • Andreas Heeren
  • Wolfgang Henschel
  • Dieter Kern
  • Karl-Heinz Wiesmüller
  • Roland Brock
Abstract
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Abstract

Drug development and cell biological research alike are confronted by the complexity of molecular signal transduction networks. These networks integrate information from different stimuli inside the cell. Progress in both disciplines will largely depend on experimental techniques that reflect the complexity of cellular signalling networks. The testing of combinations, either of drug candidates or of stimuli is considered one promising solution of this challenge. However, the systematic testing of combinations introduces a new dimension of experimental complexity. Miniaturization, parallelization and especially a reduction of the number of steps required for the preparation of samples are prerequisites for resolving this dilemma. As a solution for the testing of different combinations of drug candidates, we present the generation of substance mixtures by diffusion. For each combination of compounds only two pipetting steps are required for generating a virtually unlimited number of different test conditions. The potential of testing the response of cells to systematic combinations of different stimuli is demonstrated by using antibody microarrays where each spot carries a different combination of stimulatory antibodies. Both experimental strategies are combinatorial in nature. Through miniaturization and implementation of high-content read-outs a maximum of information is obtained with a minimum of manipulations.

Keywords

Concentration Gradient Drug Candidate Substance Mixture Cellular Signal Transduction Antibody Microarrays 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    http://www.signaling-gateway.org/data/cgi-bin/table2.cgi?cellabbr=RWGoogle Scholar
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    Dertinger, S. K., Jiang, X., Li, Z., Murthy, V. N. & Whitesides, G. M. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 12542–12547.CrossRefGoogle Scholar
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    Brock, R., Device and method for the generation of arrays of substance mixtures by diffusion, EP 1 298 433 A1, filed, published April 02, 2003.Google Scholar

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Alexander Ganser
    • 1
  • Günter Roth
    • 2
  • Karsten Köhler
    • 1
  • Thomas André
    • 1
  • Andreas Heeren
    • 3
  • Wolfgang Henschel
    • 3
  • Dieter Kern
    • 3
  • Karl-Heinz Wiesmüller
    • 2
  • Roland Brock
    • 1
  1. 1.Group of Cellular Signal Transduction, Dept. of Molecular BiologyUniversity of TübingenTübingenGermany
  2. 2.EMC microcollections GmbHTübingenGermany
  3. 3.Institute of Applied PhysicsUniversity of TübingenTübingenGermany

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