Incidence of Microsporidia in Cancer Patients
- 167 Downloads
Microsporidia are considered opportunistic pathogens as evidenced by the significant detection in immunocompromised HIV/AIDS population. Cancer patients receiving chemotherapy are considered to be immunosuppressed.
Materials and Methods
Stool samples were collected from 311 cancer patients in the Klang Valley. Each sample underwent water–ether concentration and staining with modified trichrome stain.
Results and Discussion
Sixty-eight samples were positive by oil immersion examination. Polymerase chain reaction amplification with specific primers on those samples amplified Encephalitozoon intestinalis from two of the samples and Encephalitozoon hellem from one sample.
Keywordsmicrosporidia cancer E. intestinalis E. hellem
- 3.Bendall RP, Chiodini PL. New diagnostic methods for parasitic infections. Curr Opin Infect Dis. 1993;6:318–23.Google Scholar
- 4.Botero JH, Castaño A, Montoya MN, Ocampo NE, Hurtado MI, et al. A preliminary study of the prevalence of intestinal parasites in immunocompromised patients with and without gastrointestinal manifestations. Rev Inst Med Trop Sao Paulo. 2003;45:197–200. doi: 10.1590/S0036-46652003000400004.PubMedGoogle Scholar
- 6.del Aguila C, Moura H, Soledad F, Navajas R, Lopez-Velez R, et al. In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS. J Clin Microbiol. 2001;39:1105–8. doi: 10.1128/JCM.39.3.1105-1108.2001.PubMedCrossRefGoogle Scholar
- 8.Franzen C, Müller A, Hartmann P, Hegener P, Schrappe M, et al. Polymerase chain reaction for diagnosis and species differentiation of microsporidia. Folia Parasitol (Praha). 1998;45:140–8.Google Scholar
- 16.Orenstein JM, Russo P, Didier ES, Bowers C, Bunin N, et al. Fatal pulmonary microsporidiosis due to Encephalitozoon cuniculi following allogeneic bone marrow transplantation for acute myelogenous leukemia. Ultrastruct Pathol. 2005;29:269–76. doi: 10.1080/01913120590951257.PubMedCrossRefGoogle Scholar
- 23.Visvesvara GS, Leitch GJ, da Silva AJ, Croppo GP, Moura H, et al. Polyclonal and monoclonal antibody and PCR-amplified small-subunit rRNA identification of a microsporidia, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. J Clin Microbiol. 1994;32:2760–8.PubMedGoogle Scholar
- 24.Weiss LM, Zhu X, Cali A, Tanowitz HB, Wittner M. Utility of microsporidian rRNA in diagnosis and phylogeny: a review. Folia Parasitol (Praha). 1994;41:81–90.Google Scholar