Abstract
That specific immunotherapy can inhibit cancer growth has been recognized; its efficiency is to be improved. This study aimed to inhibit lung cancer (LC) growth in a mouse model by using an LC-specific vaccination. In this study, a LC mouse model was created by adoptive transplantation with LC cells. The tumor-bearing mice were vaccinated with LC cell extracts plus adjuvant TNBS or adoptive transplantation with specific CD8+ CD196+ T cells. The results showed that the vaccination with LC extracts (LCE)/TNBS markedly inhibited the LC growth and induced CD8+ CD196+ T cells in LC tissue and the spleen. These CD8+ CD196+ T cells proliferated and produce high levels of perforin upon exposure to LCE and specifically induced LC cell apoptosis. Exposure to TNBS induced RAW264.7 cells to produce macrophage inflammatory protein-3α; the latter activated signal transducer and activator of transcription 3 and further induced perforin expression in the CD8+ CD196+ T cells. Adoptive transfer with specific CD8+ CD196+ T cells suppressed LC growth in mice. In conclusion, immunization with LC extracts and TNBS can induce LC-specific CD8+ CD196+ T cells in LC-bearing mice and inhibit LC growth.
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JZ, JL, HC, WW, XL, YW, ZW, KZ, YL, YW and HL performed experiments, analyzed data and reviewed the manuscript. LG designed the project, supervised the research and wrote the paper.
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Jian Zhang and Jing Liu are joint first authors.
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Zhang, J., Liu, J., Chen, H. et al. Specific immunotherapy generates CD8+ CD196+ T cells to suppress lung cancer growth in mice. Immunol Res 64, 1033–1040 (2016). https://doi.org/10.1007/s12026-016-8793-y
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DOI: https://doi.org/10.1007/s12026-016-8793-y