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Immunologic Research

, Volume 60, Issue 1, pp 105–111 | Cite as

Expansion of regulatory T cells from umbilical cord blood and adult peripheral blood CD4+CD25+ T cells

  • Syh-Jae Lin
  • Chun-Hao Lu
  • Dah-Chin Yan
  • Pei-Tzu Lee
  • Hsiu-Shan Hsiao
  • Ming-Ling Kuo
Article

Abstract

CD4+CD25+ regulatory T cells (Treg), if properly expanded from umbilical cord blood (UCB), may provide a promising immunotherapeutic tool. Our previous data demonstrated that UCB CD4+CD25+ T cells with 4-day stimulation have comparable phenotypes and suppressive function to that of adult peripheral blood (APB) CD4+CD25+ T cells. We further examined whether 2-week culture would achieve higher expansion levels of Tregs. UCB CD4+CD25+ T cells and their APB counterparts were stimulated with anti-CD3/anti-CD28 in the presence of IL-2 or IL-15 for 2 weeks. The cell proliferation and forkhead box P3 (FoxP3) expression were examined. The function of the expanded cells was then investigated by suppressive assay. IL-21 was applied to study whether it counteracts the function of UCB and APB CD4+CD25+ T cells. The results indicate that UCB CD4+CD25+ T cells expanded much better than their APB counterparts. IL-2 was superior to expand UCB and APB Tregs for 2 weeks than IL-15. FoxP3 expression which peaked on Day 10–14 was comparable. Most importantly, expanded UCB Tregs showed greater suppressive function in allogeneic mixed lymphocyte reaction. The addition of IL-21, however, counteracted the suppressive function of expanded UCB and APB Tregs. The results support using UCB as a source of Treg cells.

Keywords

IL-2 IL-15 IL-21 Regulatory T cells FoxP3 

Notes

Acknowledgments

We thank all the health volunteers for participating in this study. This study was supported in part by grants from National Science Council of Republic of China: NSC96-2314-B182A-042-MY2 and NSC101-2314-B-182-033 and grants from Chang Gung Memorial Hospital: CMRPG4A0052, CMRPD4A0053, CMRPD1A0172~3, and CMRPD190511~3.

Conflict of interest

The authors declare no financial or commercial conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Syh-Jae Lin
    • 1
  • Chun-Hao Lu
    • 2
  • Dah-Chin Yan
    • 1
  • Pei-Tzu Lee
    • 3
  • Hsiu-Shan Hsiao
    • 3
  • Ming-Ling Kuo
    • 2
    • 4
  1. 1.Division of Asthma, Allergy, and Rheumatology, Department of PediatricsChang Gung Memorial Hospital and College of Medicine, Chang Gung UniversityTaoyuanTaiwan
  2. 2.Graduate Institute of Biomedical Sciences, College of MedicineChang Gung UniversityTaoyuanTaiwan
  3. 3.Health Research DivisionChang Gung Memorial HospitalTaoyuanTaiwan
  4. 4.Department of Microbiology and Immunology, College of MedicineChang Gung UniversityTaoyuanTaiwan

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