Immunologic Research

, Volume 58, Issue 1, pp 40–50

Systemic immunogenicity of para-Phenylenediamine and Diphenylcyclopropenone: two potent contact allergy-inducing haptens

  • Jesper Dyrendom Svalgaard
  • Carina Særmark
  • Morten Dall
  • Karsten Buschard
  • Jeanne D. Johansen
  • Kåre Engkilde
Article

DOI: 10.1007/s12026-013-8482-z

Cite this article as:
Svalgaard, J.D., Særmark, C., Dall, M. et al. Immunol Res (2014) 58: 40. doi:10.1007/s12026-013-8482-z

Abstract

p-Phenylenediamine (PPD) and Diphenylcyclopropenone (DPCP) are two potent haptens. Both haptens are known to cause delayed-type hypersensitivity, involving a cytokine response and local infiltration of T-cell subpopulations, resulting in contact dermatitis. We investigated the systemic immune effects of PPD and DPCP, two relatively unexplored skin allergens. The dorsal sides of the ears of BALB/c mice were exposed to PPD or DPCP (0.1 % w/v or 0.01 % w/v), or vehicle alone. Mice were treated once daily for 3 days (induction period) and subsequently twice per week for 8 weeks. Local and systemic immune responses in the auricular and pancreatic lymph nodes, spleen, liver, serum, and ears were analyzed with cytokine profiling MSD, flow cytometry, and qPCR. Ear swelling increased significantly in mice treated with 1 % PPD, 0.01 % DPCP or 0.1 % DPCP, compared with vehicle treatment, indicating that the mice were sensitized and that there was a local inflammation. Auricular lymph nodes, pancreatic lymph nodes, spleen, and liver showed changes in regulatory T-cell, B-cell, and NKT-cell frequencies, and increased activation of CD8+ T cells and B cells. Intracellular cytokine profiling revealed an increase in the IFN-γ- and IL-4-positive NKT cells present in the liver following treatment with both haptens. Moreover, we saw a tendency toward a systemic increase in IL-17A. We observed systemic immunological effects of PPD and DPCP. Furthermore, concentrations too low to increase ear thickness and cause clinical symptoms may still prime the immune system. These systemic immunological effects may potentially predispose individuals to certain diseases.

Keywords

p-Phenylenediamine Diphenylcyclopropenone Contact hypersensitivity Contact allergy Invariant NKT cells Foxp3 

Abbreviations

CHS

Contact hypersensitivity

DTH

Delayed-type hypersensitivity

PPD

p-Phenylenediamine

DPCP

Diphenylcyclopropenone

AL-N

Auricular lymph nodes

PL-N

Pancreatic lymph nodes

Supplementary material

12026_2013_8482_MOESM1_ESM.pdf (364 kb)
Supplementary material 1 (PDF 363 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Jesper Dyrendom Svalgaard
    • 1
    • 2
  • Carina Særmark
    • 2
  • Morten Dall
    • 2
  • Karsten Buschard
    • 2
  • Jeanne D. Johansen
    • 2
  • Kåre Engkilde
    • 1
    • 2
  1. 1.Department of Dermato-Allergology, National Allergy Research Centre, Gentofte HospitalUniversity of CopenhagenHellerupDenmark
  2. 2.The Bartholin Institute, RigshospitaletCopenhagen BiocenterCopenhagenDenmark

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