Immunologic Research

, Volume 47, Issue 1–3, pp 172–178 | Cite as

Transcriptional target-based expression cloning of immunoregulatory molecules

  • Rebecca L. Lamason
  • Stefanie M. Lew
  • Joel L. Pomerantz
Article

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

Keywords

Expression-cloning Gene discovery NF-κB HIV-1 cDNA library 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Rebecca L. Lamason
    • 1
  • Stefanie M. Lew
    • 1
  • Joel L. Pomerantz
    • 1
  1. 1.Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in ImmunologyThe Johns Hopkins University School of MedicineBaltimoreUSA

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