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Immunologic Research

, 44:71 | Cite as

Characterization of cultured thymus tissue used for transplantation with emphasis on promiscuous expression of thyroid tissue-specific genes

  • Bin Li
  • Jie Li
  • Chia-San Hsieh
  • Laura P. Hale
  • Yi-Ju Li
  • Blythe H. Devlin
  • M. Louise Markert
Article

Abstract

Autoimmune thyroid disease occurs in some complete DiGeorge anomaly patients after thymus transplantation. This study was designed to assess the effect of culture of thymus tissue on the expression of genes involved in the development of autoimmunity. The expression of autoimmune regulator (AIRE), thyroglobulin (TG), thyroid peroxidase (TPO), and cytokeratin RNAs was examined in thymocytes and thymus tissue on the day of thymus harvest and after 14 and 21 days of culture. Immunohistochemistry was used to evaluate the cytokeratin expression in the thymus tissue. AIRE, TG, TPO, and cytokeratin mRNAs were found in harvest-day, 14-day and 21-day cultured tissues. Levels of AIRE, TG, and cytokeratin mRNAs were mostly higher after culture compared to expression on the harvest day, likely secondary to thymocyte depletion.

Keywords

Autoimmune thyroid disease Transplantation Thymus tissue Thymocyte Gene expression 

Notes

Acknowledgments

This work was supported by the National Institutes of Health (grants R01-AI47040, R01-AI 54843), and FDA (grant FD-R-002606). The statistical support was provided by the Biostatistics Core of Duke Translational Medicine Institute, funded by the Grant Number 1 UL1 RR024128-01 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bin Li
    • 1
  • Jie Li
    • 1
  • Chia-San Hsieh
    • 1
  • Laura P. Hale
    • 2
  • Yi-Ju Li
    • 3
  • Blythe H. Devlin
    • 1
  • M. Louise Markert
    • 1
    • 4
  1. 1.Department of PediatricsDuke University Medical CenterDurhamUSA
  2. 2.Department of PathologyDuke University Medical CenterDurhamUSA
  3. 3.Department of Biostatistics and Bioinformatics and the Center for Human GeneticsDuke University Medical CenterDurhamUSA
  4. 4.Department of ImmunologyDuke University Medical CenterDurhamUSA

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