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Forensic Science, Medicine, and Pathology

, Volume 11, Issue 1, pp 29–34 | Cite as

Recovery of salivary DNA from the skin after showering

  • Shane WilliamsEmail author
  • Edward Panacek
  • William Green
  • Sree Kanthaswamy
  • Christopher Hopkins
  • Cassandra Calloway
Original Article

Abstract

Purpose

After sexual assault there is a limited amount of time before the DNA evidence on the surface of the victim’s body is not recoverable. During an assault, the offender may leave saliva on the victim’s skin. Traditional examination methods use a swabbing technique to collect saliva for DNA testing. Victim activity, especially hygiene activity such as showering, may negatively affect DNA recovery.

Methods

In this experiment, we compared two techniques for recovery of salivary DNA from the skin’s surface after a victim showers. We compared the traditional swabbing method to a “wet-vacuum” method using the M-Vac© to collect saliva from four body regions (neck, arm, stomach, and leg). In our research, we tested whether either collection technique obtained enough salivary DNA for autosomal and Y-STR analysis. In addition, we tested whether the M-Vac© is more effective at collecting DNA from large surface areas than traditional methods, by determining the amount of DNA collected.

Results

With both collection techniques, we were able to obtain male salivary DNA from at least one body region of the female after she had showered. There was no statistical difference in the amount of DNA collected between the swabbing technique and the M-Vac©. Autosomal STR analysis failed to detect the male contributor’s DNA; therefore, we used Y-STRs. With Y-STR analysis, 47 samples returned a full male profile, and 26 samples returned a partial male profile after sample concentration.

Conclusions

This research shows that salivary DNA can be collected from skin after showering and successfully analyzed using Y-STRs.

Keywords

Sexual assault M-Vac Y-STRs DNA collection 

Notes

Acknowledgments

The authors would like to thank Christopher Hopkins, Marc Wander, Katie Caswell, and Wayne Carlson for scientific and technical support.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Shane Williams
    • 1
    Email author
  • Edward Panacek
    • 1
  • William Green
    • 3
  • Sree Kanthaswamy
    • 1
  • Christopher Hopkins
    • 1
  • Cassandra Calloway
    • 1
    • 2
  1. 1.Forensic Science Graduate ProgramUniversity of California, DavisDavisUSA
  2. 2.Children’s Hospital Oakland Research InstituteOaklandUSA
  3. 3.California Clinical Forensic Medical Training CenterSacramentoUSA

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