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Neuroinformatics

, Volume 12, Issue 4, pp 535–542 | Cite as

Validation of FreeSurfer-Estimated Brain Cortical Thickness: Comparison with Histologic Measurements

  • Francesco CardinaleEmail author
  • Giuseppa Chinnici
  • Manuela Bramerio
  • Roberto Mai
  • Ivana Sartori
  • Massimo Cossu
  • Giorgio Lo Russo
  • Laura Castana
  • Nadia Colombo
  • Chiara Caborni
  • Elena De Momi
  • Giancarlo Ferrigno
Original Article

Abstract

FreeSurfer software package automatically estimates the cerebral cortical thickness. Its use is widely accepted, albeit this tool was validated against histologic measurements in only two post-mortem isolated brain MR scans. Indeed, a comparison between histologic measurements and FreeSurfer estimation from in vivo data was never performed. At the “Claudio Munari” Center for Epilepsy and Parkinson Surgery we have included FreeSurfer in our presurgical workflow since 2008, mainly because the automatic reconstruction of the brain surface is useful for carefully planning the surgical resection. We therefore compared cortical thickness values obtained by the automatic software pipeline with manual histologic measurements performed on 27 histologic specimens resected from the corresponding brain regions of the same epileptic subjects. This method-comparison study, including Passing–Bablok regression and Bland-Altman plot analysis, showed a good agreement between FreeSurfer estimation and histologic measurements of cortical thickness. The mean cortical thickness values (±Standard Deviation) obtained with FreeSurfer and histologic measurements were 3.65 mm ± 0.44 and 3.72 mm ± 0.36, respectively (P value = 0.32). Our findings strengthen previous reports on cortical thickness changes as biomarkers of different neurological conditions.

Keywords

Cortical thickness FreeSurfer Histologic measurement Epilepsy surgery Bland-Altman Passing–Bablok 

Notes

Acknowledgments

We would like to thank Roberto Spreafico for helping us in editing the Materials and Methods section, and Steve Gibbs for reviewing the report. Moreover, we would like to thank Gianfranco De Gregori and his coworkers for their invaluable contribution to bibliographic research.

Disclosures

The Authors have nothing to disclose.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Francesco Cardinale
    • 1
    Email author
  • Giuseppa Chinnici
    • 2
  • Manuela Bramerio
    • 3
  • Roberto Mai
    • 1
  • Ivana Sartori
    • 1
  • Massimo Cossu
    • 1
  • Giorgio Lo Russo
    • 1
  • Laura Castana
    • 1
  • Nadia Colombo
    • 4
  • Chiara Caborni
    • 2
  • Elena De Momi
    • 2
  • Giancarlo Ferrigno
    • 2
  1. 1.“Claudio Munari” Center for Epilepsy and Parkinson SurgeryNiguarda HospitalMilanItaly
  2. 2.Nearlab, Bioengineering DepartmentPolitecnico di MilanoMilanItaly
  3. 3.Service of PathologyNiguarda HospitalMilanItaly
  4. 4.Department of NeuroradiologyNiguarda HospitalMilanItaly

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