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Neuroinformatics

, Volume 9, Issue 2–3, pp 159–166 | Cite as

Neuronal Tracing for Connectomic Studies

  • Ju Lu
Mini-Review
First Online:

Abstract

Reconstruction of the complete wiring diagram, or connectome, of a neural circuit provides an alternative approach to conventional circuit analysis. One major obstacle of connectomics lies in segmenting and tracing neuronal processes from the vast number of images obtained with optical or electron microscopy. Here I review recent progress in automated tracing algorithms for connectomic reconstruction with fluorescence and electron microscopy, and discuss the challenges to image analysis posed by novel optical imaging techniques.

Keywords

Connectomics Image segmentation Neuron tracing Fluorescence microscopy Electron microscopy 
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Notes

Acknowledgement

The author thanks Prof. Mark Schnitzer for support of this work; Prof. Giorgio Ascoli, Prof. Jeff W. Lichtman, Prof. Yi Zuo, and the two anonymous reviewers for discussions and comments on this manuscript.

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© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.James H. Clark Center for Biomedical Engineering and Sciences, Department of Biological SciencesStanford UniversityStanfordUSA

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