In early pubertal boys, testosterone and LH are associated with improved anti-oxidation during an aerobic exercise bout
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To investigate the association of the hypothalamic-pituitary-testicular (HPT) axis with pro- and anti- oxidation, in relation to puberty and obesity in boys, before and after an aerobic exercise bout.
This is a cross-sectional human observational study of 92 healthy normal-weight, obese pre- and early- pubertal boys that underwent a blood sampling, before, and after an aerobic exercise bout at 70% VO2max, until exhaustion. LH, FSH, total testosterone (tT) and markers of pro- (TBARS and PCs) and anti- (GSH, GSSG, GPX, catalase, TAC) oxidation were measured.
Baseline LH, FSH, and tT concentrations were greater in early, than in pre- pubertal boys, independently of weight status. Post-exercise, LH concentrations decreased in early pubertal boys while FSH concentrations did not change in any of the studied groups. Baseline and post-exercise tT concentrations were lower in obese than in normal-weight early pubertal boys, while baseline and post-exercise LH and FSH concentrations did not differ between these groups. Post-exercise tT concentrations increased in early pubertal obese boys. Baseline LH, FSH and tT concentrations correlated positively with baseline anti-oxidation markers concentrations in pre-pubertal boys. Baseline tT concentrations correlated positively with the increase of TAC concentrations in early pubertal normal-weight boys. In all boys, baseline LH concentrations were the best positive predictors for the exercise-associated increase of TAC concentrations.
It appears that the HPT axis maturation during puberty (in particular its LH and testosterone components) is positively associated with the increase of anti-oxidation during a bout of aerobic exercise.
KeywordsHPT axis obesity puberty LH, FSH, testosterone
This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors. All procedures performed in the study involving human participants had the approval of the National and Kapodistrian University of Athens Medical School designated ethics committee and were in accordance with the 1964 Helsinki declaration and its later amendments.
The aims and the procedure of the study were fully disclosed to the parents or legal guardians of the participants, and informed, written consent was obtained from them while boys gave verbal consent before the participation in the study.
- 4.S. Vandewalle, Y. Taes, T. Fiers, M. Van Helvoirt, P. Debode, N. Herregods, C. Ernst, E. Van Caenegem, I. Roggen, F. Verhelle, J. De Schepper, J.M. Kaufman, Sex steroids in relation to sexual and skeletal maturation in obese male adolescents. J. Clin. Endocrinol. Metab. 99(8), 2977–2985 (2014). https://doi.org/10.1210/jc.2014-1452 CrossRefPubMedGoogle Scholar
- 7.R.J. Alleman, L.A. Katunga, M.A. Nelson, D.A. Brown, E.J. Anderson, The “Goldilocks Zone” from a redox perspective-Adaptive vs. deleterious responses to oxidative stress in striated muscle. Front. Physiol. 5, 358 (2014). https://doi.org/10.3389/fphys.2014.00358 CrossRefPubMedPubMedCentralGoogle Scholar
- 8.G. Paltoglou, I.G. Fatouros, G. Valsamakis, M. Schoina, A. Avloniti, A. Chatzinikolaou, A. Kambas, D. Draganidis, A. Mantzou, M. Papagianni, C. Kanaka-Gantenbein, G.P. Chrousos, G. Mastorakos, Anti-oxidation improves in puberty in normal weight and obese boys, in positive association with exercise-stimulated growth hormone secretion. Pediatr. Res. (2015). pr201585 [pii] https://doi.org/10.1038/pr.2015.85 CrossRefGoogle Scholar
- 9.A. Avloniti, A. Chatzinikolaou, C.K. Deli, D. Vlachopoulos, L. Gracia-Marco, D. Leontsini, D. Draganidis, A.Z. Jamurtas, G. Mastorakos, I.G. Fatouros, Exercise-Induced Oxidative Stress Responses in the Pediatric Population. Antioxidants (Basel) 6(1) (2017). E6 [pii] https://doi.org/10.3390/antiox6010006 antiox6010006 [pii]CrossRefGoogle Scholar
- 12.A. Mancini, E. Leone, R. Festa, G. Grande, A. Silvestrini, L. de Marinis, A. Pontecorvi, G. Maira, G.P. Littarru, E. Meucci, Effects of testosterone on antioxidant systems in male secondary hypogonadism. J. Androl. 29(6), 622–629 (2008). jandrol.107.004838 [pii] https://doi.org/10.2164/jandrol.107.004838 CrossRefPubMedGoogle Scholar
- 14.G. Mastorakos, M. Pavlatou, E. Diamanti-Kandarakis, G.P. Chrousos, Exercise and the stress system. Hormone 4(2), 73–89 (2005)Google Scholar
- 15.G. Gerra, R. Caccavari, N. Reali, P. Bonvicini, A. Marcato, G. Fertonani, R. Delsignore, M. Passeri, F. Brambilla, Noradrenergic and hormonal responses to physical exercise in adolescents. Relationship to anxiety and tolerance to frustration. Neuropsychobiology 27(2), 65–71 (1993). 118955 [pii] 118955CrossRefGoogle Scholar
- 17.L. Di Luigi, C. Baldari, M.C. Gallotta, F. Perroni, F. Romanelli, A. Lenzi, L. Guidetti, Salivary steroids at rest and after a training load in young male athletes: relationship with chronological age and pubertal development. Int. J. Sports. Med. 27(9), 709–717 (2006). https://doi.org/10.1055/s-2005-872931 CrossRefPubMedGoogle Scholar
- 19.W.J. Kraemer, K. Hakkinen, R.U. Newton, B.C. Nindl, J.S. Volek, M. McCormick, L.A. Gotshalk, S.E. Gordon, S.J. Fleck, W.W. Campbell, M. Putukian, W.J. Evans, Effects of heavy-resistance training on hormonal response patterns in younger vs. older men. J. Appl. Physiol. 87(3), 982–992 (1999). https://doi.org/10.1152/jappl.1922.214.171.1242 CrossRefPubMedGoogle Scholar
- 21.D. Chiotis, X. Krikos, G. Tsiftis, M. Hatzisymeon, M. Maniati-Christidi, A. Dacou-Voutetaki, Body mass index and prevalence of obesity in subjects of Hellenic origin aged 0–18 years living in the Athens area. Ann. Clin. Pediatr. Unive. Atheniensis. (51), 139–154 (2004)Google Scholar
- 24.K. Albertsson-Wikland, S. Rosberg, B. Lannering, L. Dunkel, G. Selstam, E. Norjavaara, Twenty-four-hour profiles of luteinizing hormone, follicle-stimulating hormone, testosterone, and estradiol levels: a semilongitudinal study throughout puberty in healthy boys. J. Clin. Endocrinol. Metab. 82(2), 541–549 (1997). https://doi.org/10.1210/jcem.82.2.3778 CrossRefPubMedGoogle Scholar
- 25.A.S. Kelly, J. Steinberger, T.P. Olson, D.R. Dengel, In the absence of weight loss, exercise training does not improve adipokines or oxidative stress in overweight children. Metabolism 56(7), 1005–1009 (2007). S0026-0495(07)00108-4 [pii] https://doi.org/10.1016/j.metabol.2007.03.009 CrossRefPubMedGoogle Scholar
- 26.E. von Elm, D.G. Altman, M. Egger, S.J. Pocock, P.C. Gotzsche, J.P. Vandenbroucke, The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) Statement: guidelines for reporting observational studies. Int. J. Surg. 12(12), 1495–1499 (2014). https://doi.org/10.1016/j.ijsu.2014.07.013. S1743-9191(14)00212-X [pii]CrossRefGoogle Scholar
- 28.G. Paltoglou, M. Schoina, G. Valsamakis, N. Salakos, A. Avloniti, A. Chatzinikolaou, A. Margeli, C. Skevaki, M. Papagianni, C. Kanaka-Gantenbein, I. Papassotiriou, G.P. Chrousos, I.G. Fatouros, G. Mastorakos, Interrelations among the adipocytokines leptin and adiponectin, oxidative stress and aseptic inflammation markers in pre- and early-pubertal normal-weight and obese boys. Endocrine 55(3), 925–933 (2017). [pii] https://doi.org/10.1007/s12020-017-1227-3 CrossRefGoogle Scholar
- 32.J.D. Veldhuis, A.D. Rogol, E. Samojlik, N.H. Ertel, Role of endogenous opiates in the expression of negative feedback actions of androgen and estrogen on pulsatile properties of luteinizing hormone secretion in man. J. Clin. Invest. 74(1), 47–55 (1984). https://doi.org/10.1172/JCI111417 CrossRefPubMedPubMedCentralGoogle Scholar
- 34.J.L. Crawford, J.R. McNeilly, L. Nicol, A.S. McNeilly, Promotion of intragranular co-aggregation with LH by enhancement of secretogranin II storage resulted in increased intracellular granule storage in gonadotrophs of GnRH-deprived male mice. Reproduction 124(2), 267–277 (2002)CrossRefGoogle Scholar
- 41.C. Enea, N. Boisseau, M. Ottavy, J. Mulliez, C. Millet, I. Ingrand, V. Diaz, B. Dugue, Effects of menstrual cycle, oral contraception, and training on exercise-induced changes in circulating DHEA-sulphate and testosterone in young women. Eur. J. Appl. Physiol. 106(3), 365–373 (2009). https://doi.org/10.1007/s00421-009-1017-6 CrossRefPubMedGoogle Scholar
- 47.D. Benitez-Sillero Jde, J.L. Perez-Navero, I. Tasset, M. Guillen-Del Castillo, M. Gil-Campos, I. Tunez, Influence of intense exercise on saliva glutathione in prepubescent and pubescent boys. Eur. J. Appl. Physiol. 106(2), 181–186 (2009). https://doi.org/10.1007/s00421-009-1004-y CrossRefPubMedGoogle Scholar