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Endocrine

, Volume 66, Issue 2, pp 266–277 | Cite as

Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity

  • María Alejandra Paulazo
  • Alicia Juana Klecha
  • Helena Andrea Sterle
  • Eduardo Valli
  • Horacio Torti
  • Florencia Cayrol
  • María Laura Barreiro Arcos
  • Graciela Alicia CremaschiEmail author
Original Article
  • 133 Downloads

Abstract

Purpose

Hypothyroidism has been shown to induce immunosuppression and both the thyroid status and immunity are affected by zinc deficiency. However, the impact of hypothyroidism on zinc metabolism and its possible relationship with the immune status has not yet been deeply explored. Here, our aim was to study whether hypothyroidism may alter zinc metabolism and thus lead to the impairment of T lymphocyte activity.

Methods

Variations in the distribution of zinc in the body were evaluated in PTU-treated hypothyroid mice. The effects of hypothyroidism and zinc deficiency were studied on T lymphocyte proliferation after stimulation both in vitro and in vivo. For in vitro assays, thyroid hormone-free or zinc chelator (TPEN or DTPA)-supplemented media were used. For in vivo assays, lymphocyte activity was evaluated in cells from hypothyroid, T3-treated, and zinc-supplemented mice.

Results

Hypothyroid mice showed lower levels of zinc in femur and lymph nodes than controls. T3 and zinc supplementation reversed these effects. In vitro, both thyroid hormone and zinc deficiency led to a decreased response to mitogen stimulation. However, only zinc deficiency was able to induce lymphocyte apoptosis. Mitogen-stimulated T cells from hypothyroid mice showed impaired proliferation, accompanied by decreased activation of PKC and lower levels of p-ERK, effects that were reversed by T3 replacement or zinc supplementation.

Conclusions

Our results show an important role of zinc deficiency in hypothyroid-mediated T-cell suppression and suggest the importance of evaluating zinc levels and restoring them when necessary to maintain an efficient immune response in hypothyroid patients.

Keywords

Hypothyroidism Zinc Thyroid hormones T lymphocytes 

Notes

Acknowledgements

This work was supported by the National Research Council of Argentina (PIP-CONICET 00275), the University of Buenos Aires (UBACYT 20020130100289BA), the National Agency for Science and Technology (ANPCYT, PICT 2015–0874)

Funding

This study was funded by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Argentina, PIP-CONICET 00275; the Universidad de Buenos Aires, UBACYT 20020130100289BA and the Fondo para la Investigación Científica y Tecnológica (FONCYT) PICT 2015-0874.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All studies using animals were performed in accordance with the ARRIVE Guidelines [21] and all the experimental protocols were approved by the Institutional Committee for the Care and Use of Laboratory Animals (CICUAL) of the School of Pharmacy and Biochemistry of the University of Buenos Aires and the CICUAL of BIOMED. This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

12020_2019_1936_MOESM1_ESM.docx (13 kb)
Supplementary Figure S1.
12020_2019_1936_MOESM2_ESM.TIF
Supplementary Figure S1.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • María Alejandra Paulazo
    • 1
  • Alicia Juana Klecha
    • 1
    • 2
  • Helena Andrea Sterle
    • 1
  • Eduardo Valli
    • 1
  • Horacio Torti
    • 2
  • Florencia Cayrol
    • 1
  • María Laura Barreiro Arcos
    • 1
  • Graciela Alicia Cremaschi
    • 1
    • 2
    Email author
  1. 1.Instituto de Investigaciones Biomédicas (BIOMED), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias MédicasPontificia Universidad Católica Argentina (UCA)Buenos AiresArgentina
  2. 2.Cátedra de Física, Facultad de Farmacia y BioquímicaUniversidad de Buenos AiresCiudad Autónoma de Buenos AiresArgentina

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