, Volume 62, Issue 2, pp 274–280 | Cite as

Evaluation of testicular function in prepubertal children

  • Rosita A. Condorelli
  • Rossella Cannarella
  • Aldo E. Calogero
  • Sandro La VigneraEmail author



The assessment of testicular function is not currently performed in childhood. The aim of this review was to address the usefulness of serum Anti-Müllerian Hormone (AMH), inhibin B, and testicular volume (TV) evaluation in children.


Serum AMH and inhibin B levels reflect Sertoli cells (SCs) health and number, SCs maturation degree and their exposure to FSH and to intra-tubular testosterone (T). These hormones might be helpful in discriminating between congenital central hypogonadism (cHH) and constitutional delay in growth and puberty (CDGP) and in case of clinical suspicious of precocious puberty. Furthermore, low AMH and/or inhibin B levels have been observed in children with primary testicular disorders, suggesting the existence of SC dysfunction. TV also provides useful information on testicular health. Recently, a medical calculator, requiring testis length and the stage of genital development, has been developed to easily derive TV.


The evidence supports the usefulness of AMH, inhibin B and TV evaluation for the early diagnosis of puberty disorders and primary testicular damage. We suggest the measurement of TV by using the medical calculator in all children and to reserve AMH and inhibin B measurements to those cases of no testicular growth, clinical suspicious of puberty disorders or in children at risk for spermatogenesis damage. This work-up might allow the early detection of testicular tubular damage which, in turn, may be useful to prevent the oncoming male infertility in adulthood.


AMH Anti-Müllerian hormone Inhibin B Sertoli cells Pre-pubertal children Testicular function 



Anti-Müllerian hormone


congenital hypogonadotropichypogonadism


Follicle-stimulating hormone


Gonadotropin-realizing hormone


Gonadotropin-realizing hormone antagonist


luteinizing hormone


Sertoli cells




Testicular distance to pubic bone


Compliance with ethical standards

Conflict of interest

The author declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rosita A. Condorelli
    • 1
  • Rossella Cannarella
    • 1
  • Aldo E. Calogero
    • 1
  • Sandro La Vignera
    • 1
    Email author
  1. 1.Department of Clinical and Experimental MedicineUniversity of Catania, Policlinico “G. Rodolico”CataniaItaly

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