, Volume 50, Issue 2, pp 326–334 | Cite as

Effect of 1-year dietary supplementation with vitaminized olive oil on markers of bone turnover and oxidative stress in healthy post-menopausal women

  • Laura MazzantiEmail author
  • Maurizio Battino
  • Laura Nanetti
  • Francesca Raffaelli
  • Alessandro Alidori
  • Giulia Sforza
  • Flavia Carle
  • Veronica Quagliarini
  • Nelvio Cester
  • Arianna Vignini
Endocrine Trials


Osteoporosis represents a serious health problem worldwide associated with an increased risk of fractures and mortality. Nutrition should form part of bone disease prevention strategies, especially in the light of the population ageing and the diet effect on bone health. Thus the study aimed at verifying whether 1 year of oral supplementation with either extra virgin olive oil (VOO) enriched with vitamins D3, K1 and B6 (VitVOO) or VOO used as placebo (PlaVOO) is able to modify some bone turnover and oxidative stress markers. Bone mineral density (BMD) was assessed in 60 healthy post-menopausal women together with the bone vitamin K status by measuring undercarboxylated osteocalcine (ucOC) plasma levels, the ratio between ucOC and carboxylated osteocalcine (UCR) and the relations with oxidative stress markers. After 1 year (T 1), subjects taking VitVOO showed lower ucOC levels than those taking PlaVOO; the same trend was found for UCR. As far as BMD is concerned, a significant increase in T-score at T 1 in VitVOO subjects compared to PlaVOO was found. All oxidative stress markers as thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes showed a significant reduction after VitVOO supplementation, whilst plasma total antioxidant capacity values was significantly increased in VitVOO group compared to PlaVOO group at T 1. It might be suggested that the use of VitVOO in the diet of post-menopausal women could represent a proper tool for bone protection and a useful strategy against oxidative stress and related diseases, thus confirming the antioxidant role played by the added vitamins.


Vitaminized oil Osteoporosis Oxidative stress Osteocalcin Bone mineral density 



Authors wish to thank Dr Francesca Petrini and Fattoria Petrini, Via San Vito, 12-60037 Monte San Vito (AN) for financial support, and Dr Luca Tiano for technical support on osteocalcin determination.

Conflict of interest

The authors of this research article do not have any conflict of interest at the time of submission.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Laura Mazzanti
    • 1
    • 4
    Email author
  • Maurizio Battino
    • 1
  • Laura Nanetti
    • 1
  • Francesca Raffaelli
    • 1
  • Alessandro Alidori
    • 1
  • Giulia Sforza
    • 1
  • Flavia Carle
    • 2
  • Veronica Quagliarini
    • 3
  • Nelvio Cester
    • 3
  • Arianna Vignini
    • 1
  1. 1.Section of Biochemistry, Biology and Physics, Department of Clinical Sciences, School of NutritionUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Department of Epidemiology and Biostatistics, School of MedicineUniversità Politecnica delle MarcheAnconaItaly
  3. 3.Department of Obstetrics, Gynecology and PediatricsSenigallia Hospital - ASLSenigalliaItaly
  4. 4.Section of Biochemistry, Department of Clinical Science, School of MedicineUniversità Politecnica delle MarcheAnconaItaly

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