Endocrine

, Volume 43, Issue 3, pp 705–713

Altered testicular development as a consequence of increase number of sertoli cell in male lambs exposed prenatally to excess testosterone

  • Pedro P. Rojas-García
  • Mónica P. Recabarren
  • Teresa Sir-Petermann
  • Rodolfo Rey
  • Sergio Palma
  • Albert Carrasco
  • Carlos C. Perez-Marin
  • Vasantha Padmanabhan
  • Sergio E. Recabarren
Original Article

DOI: 10.1007/s12020-012-9818-5

Cite this article as:
Rojas-García, P.P., Recabarren, M.P., Sir-Petermann, T. et al. Endocrine (2013) 43: 705. doi:10.1007/s12020-012-9818-5

Abstract

The reprograming effects of prenatal testosterone (T) treatment on postnatal reproductive parameters have been studied extensively in females of several species but similar studies in males are limited. We recently found that prenatal T treatment increases Sertoli cell number and reduced spermatogenesis in adult rams. If such disruptions are manifested early in life and involve changes in testicular paracrine environment remain to be explored. This study addresses the impact of prenatal T excess on testicular parameters in infant males, including Sertoli cell number and expression of critical genes [FSH receptor (FSHR), androgen receptor (AR), transforming growth factor beta 1 (TGFB1), 3 (TGFB3), transforming growth factor beta type 1 receptor, (TGFBR1), and anti-Müllerian hormone (AMH)] modulating testicular function. At 4 week of age, male lambs born to dams treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T-males), had a higher number of Sertoli cells/testis (P = 0.035) than control males (C-males) born to dams treated with the vehicle. While no differences were observed in the expression of FSHR and TGFB3, testicular TGFBR1 expression was found to be lower in T-males (P = 0.03) compared to C-males. Expression level of AMH, TGFB1, and AR also tended to be lower in T-males. These findings provide evidence that impact of fetal exposure to T excess is evident early in postnatal life, mainly characterized by an increase in Sertoli cell number. This could explain the testicular dysfunction observed in adult rams.

Keywords

Developmental programing Ovine Male reproduction Testes 

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Pedro P. Rojas-García
    • 1
  • Mónica P. Recabarren
    • 1
  • Teresa Sir-Petermann
    • 2
  • Rodolfo Rey
    • 3
  • Sergio Palma
    • 1
  • Albert Carrasco
    • 1
  • Carlos C. Perez-Marin
    • 4
  • Vasantha Padmanabhan
    • 5
  • Sergio E. Recabarren
    • 1
  1. 1.Laboratory of Animal Physiology and Endocrinology, Faculty of Veterinary SciencesUniversity of ConcepciónChillánChile
  2. 2.Laboratory of Endocrinology and Metabolism, Department of Internal Medicine, Western Faculty of MedicineUniversity of ChileCorreo 33, SantiagoChile
  3. 3.Center for Research in Endocrinology Children’s Hospital “R. Gutiérrez” C1425EFD Buenos Aires, Argentina, and Department of Histology, Cell Biology, EmbryologyUniversity of Buenos AiresBuenos AiresArgentina
  4. 4.Department of Animal Medicine and Surgery, Faculty of Veterinary MedicineUniversity of CordobaCordobaSpain
  5. 5.Departments of Pediatrics and the Reproductive Sciences ProgramUniversity of MichiganAnn ArborUSA

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