Endocrine

, Volume 43, Issue 2, pp 360–369 | Cite as

Ginkgo biloba L. extract enhances the effectiveness of syngeneic bone marrow mesenchymal stem cells in lowering blood glucose levels and reversing oxidative stress

  • Ming Ren
  • Shujuan Yang
  • Jianhui Li
  • Yulin Hu
  • Zhixing Ren
  • Shuping Ren
Original Article
First Online:
Received:
Accepted:

Abstract

Bone marrow mesenchymal stem cells (BMSCs) are potential therapy for diabetes. Owing to the oxidative stress caused by hyperglycemia, these transplanted BMSCs are with high rate of apoptotic death after transplantation. Ginkgo biloba L. extract (EGB) is a potent antioxidant which can remove free radicals. The study was to investigate whether EGB can protect BMSCs from oxidative stress in vitro and enhance the efficacy of BMSCs in lowering blood glucose levels after transplantation. BMSCs were cultured with H2O2, EGB, or H2O2 and EGB. Cell viability, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and cell death rates were determined. Diabetes was induced by single injection of streptozotocin (STZ) in male Wistar rats. Diabetic rats received EGB, BMSCs, or EGB/BMSCs. The serum levels of glucose, insulin, interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), MDA, SOD, and GSH-Px were determined. PKCα expression and NF-κB activation in kidney were determined. The MDA levels and cell death rates in BMSCs cultured with H2O2 and EGB were significantly lower; cell viability, SOD, and GSH-Px activities were significantly higher compared with those with H2O2 alone. Compared with diabetic rats receiving BMSCs, diabetic rats receiving EGB before BMSCs transplantation showed (1) significantly lower levels of blood glucose, serum MDA, IL-6, and TNF-α, and higher levels of insulin, SOD, and GSH-Px activities; (2) significantly lower PKCα expression and NF-κB activation in the kidney. EGB administration before BMSC transplantation can enhance the effectiveness of BMSCs in lowering blood glucose levels and reversing oxidative stress in diabetic rats.

Keywords

Bone marrow mesenchymal stem cells Ginkgo biloba L. extract Diabetes mellitus Antioxidant Oxidative stress 
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Notes

Acknowledgments

We would like to thank Dr Lorann Stallones (Colorado Injury Control Research Center, Colorado State University) for revising the manuscript for us. This study was supported by the Department of Science and Technology of Jilin Province, China (Grant No 201105019).

Conflict of interest

The authors declare that there is no conflict of interest associated with this manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ming Ren
    • 1
  • Shujuan Yang
    • 1
  • Jianhui Li
    • 1
  • Yulin Hu
    • 2
  • Zhixing Ren
    • 1
  • Shuping Ren
    • 1
  1. 1.Department of Toxicology, School of Public HealthJilin UniversityChangchunChina
  2. 2.Department of Liver, Gallbladder and PancreasFirst Hospital of Jilin UniversityChangchunChina

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