Obesidomics: contribution of adipose tissue secretome analysis to obesity research
- 872 Downloads
Obesity is presently reaching pandemic proportions and it is becoming a major health concern in developed and developing countries due to its comorbidities like type II diabetes, cardiovascular pathologies, and some cancers. The discovery of the adipose tissue role as an endocrine gland able to secrete adipokines that affects whole-body energy homeostasis has become a key break-through toward a better molecular understanding of obesity. Among the known adipokines involved in the regulation of energy metabolism very few have been clearly seen as central regulators of insulin sensitivity, metabolism, and energy homeostasis. Thus, the discovery and characterization of new adipocyte-derived factors is still in progress. Proteomics technology has emerged as a useful tool to analyze adipose tissue secretion (secretome) dynamics giving a wider picture into the molecular events that control body weight. Besides the identification of new secreted proteins, the advantage of using this approach is the possibility to detect post-translational modifications and protein interactions that generally cannot be predicted by genome studies. In this review, we summarize the recent efforts to identify new bioactive adipokines by proteomics especially in pathological situations such as obesity.
KeywordsSecretome Proteomics Obesity Adipose tissue
This study has been funded by Instituto de Salud Carlos III (ISCIII: Ministerio de Ciencia e Innovación de España): FIS PI0/00537 (FEDER co-financing); FIS PS09/02075; CP08/00216; Xunta de Galicia 10PXIB918273PR. A.R–R is funded by CIBER FisiopatologíaObesidad y Nutrición (CB06/03); M.P. is a Miguel Servet Fellow (Instituto de Salud Carlos III/SERGAS).L.M.Seoane is a ISCIII/SERGAS Researcher.
- 8.N. Halberg, I. Wernstedt-Asterholm, and P.E. Scherer, The adipocyte as an endocrine cell. Endocrinol. Metab. Clin. North. Am. 37, 753–68, x–xi (2008)Google Scholar
- 11.M. Blüher, Vaspin in obesity and diabetes: pathophysiological and clinical significance. Endocrine (2011) (in press)Google Scholar
- 15.M.P. et al. Lodish G, Berk A, Zipursky SL. Mol. Cell Biol. New York (1999)Google Scholar
- 19.N. Aoki, S. Jin-no, Y. Nakagawa, N. Asai, E. Arakawa, N. Tamura, T. Tamura, T. Matsuda, Identification and characterization of microvesicles secreted by 3T3-L1 adipocytes: redox- and hormone-dependent induction of milk fat globule-epidermal growth factor 8-associated microvesicles. Endocrinology 148, 3850–3862 (2007)PubMedCrossRefGoogle Scholar
- 22.M. Bodén and J. Hawkins, Prediction of subcellular localization using sequence-biased recurrent networks. Bioinformatics (Oxford, England) 21, 2279–2286 (2005)Google Scholar
- 28.S. Yang, C.-M., Huang, Recent advances in protein profiling of tissues and tissue fluids. Expert Rev. Proteomics. 4, 515–529 (2007)Google Scholar
- 35.S. Famulla, D. Lamers, S. Hartwig, W. Passlack, A. Horrighs, A. Cramer, S. Lehr, H. Sell, J. Eckel, Pigment epithelium-derived factor (PEDF) is one of the most abundant proteins secreted by human adipocytes and induces insulin resistance and inflammatory signaling in muscle and fat cells. Int. J. Obes. 35, 762–772 (2011)CrossRefGoogle Scholar
- 36.D. Lamers, S. Famulla, N. Wronkowitz, S. Hartwig, S. Lehr, D.M. Ouwens, K. Eckardt, J.M. Kaufman, M. Ryden, S. Müller, F.-G. Hanisch, J. Ruige, P. Arner, H. Sell, J. Eckel, Dipeptidyl peptidase 4 is a novel adipokine potentially linking obesity to the metabolic syndrome. Diabetes 60, 1917–1925 (2011)PubMedCrossRefGoogle Scholar
- 50.J.R. Peinado, Y. Jimenez-Gomez, M.R. Pulido, M. Ortega-Bellido, C. Diaz-Lopez, F.J. Padillo, J. Lopez-Miranda, R. Vazquez-Martínez, M.M. Malagón, The stromal-vascular fraction of adipose tissue contributes to major differences between subcutaneous and visceral fat depots. Proteomics 10, 3356–3366 (2010)PubMedCrossRefGoogle Scholar
- 51.R. Pérez-Pérez, F.J. Ortega-Delgado, E. García-Santos, J.A. López, E. Camafeita, W. Ricart, J.-M. Fernández-Real, B. Peral, Differential proteomics of omental and subcutaneous adipose tissue reflects their unalike biochemical and metabolic properties. J. Proteome Res. 8, 1682–1693 (2009)PubMedCrossRefGoogle Scholar