, Volume 40, Issue 3, pp 462–471 | Cite as

Biochemical and histological liver changes occurred after iron supplementation and possible remediation by garlic consumption

  • Héla Ghorbel
  • Ines Feki
  • Ines Friha
  • Abdel Majid Khabir
  • Tahya Boudawara
  • Mohamed Boudawara
  • Sami Sayadi
Original Article


Iron liver excess is associated to biochemical and histological liver perturbations. Our aim was to know even if fresh garlic consumption can remediate these problems. Three groups of rats were utilized: control group A, iron overload group B and garlic and iron overload group C. Important morphological and biochemical modifications were obtained in group B rats comparatively to control group A. Indeed, body and liver weights and liver iron contents increased, respectively, by 12.5 ± 0.06%; 17 ± 0.25% and 35 ± 0.11% comparatively to controls. Radical cation scavenging ability in liver cytosol of group B rats was significantly low (54 ± 0,1%) in comparison to group A. Garlic consumption allowed the group C to achieve an increase by 46 ± 0,11 and 75 ± 0,14% of total antioxidant capacity comparatively to group A and B rats. For the serum ALAT, ASAT, triglyceride and LDH levels, they increased in iron-treated rats, respectively, by 25 ± 0.21; 15 ± 0.12; 30 ± 0.14 and 22 ± 0.16% comparatively to controls. These perturbations were accompanied by deep histological changes. After food fresh garlic supplementation, we had found a deep regulation of all modified parameters showing a hepatoprotective effect of garlic against iron liver excess. Garlic chemical compounds have curative effects on iron liver excess.


Liver Iron Garlic Rat Histopathology ASAT ALAT Triglyceride Total antioxidant capacity 



The authors are indebted to Mrs Ines RGAIG, Miss Najet MEZGHANNI, Mr Slim LOUKIL and Mr Adel GARGOUBI for their skilful technical assistance. This research was supported by the Laboratory of Physiology in Superior Institute of Biotechnology of Sfax Tunisia and the Laboratory of Environnemental Bioproceeds in Center of Biotechnology by EEC contract ICA3-CT2002-10033, and the ‘Contracts Programmes SERST’, Tunisia.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Héla Ghorbel
    • 1
  • Ines Feki
    • 1
  • Ines Friha
    • 1
  • Abdel Majid Khabir
    • 2
  • Tahya Boudawara
    • 2
  • Mohamed Boudawara
    • 3
  • Sami Sayadi
    • 1
  1. 1.Laboratoire des Bioprocédés Environnemtaux, Pôle d’excellence régional AUF (PER-LBP), Center de biotechnologie de Sfax (CBS)Université de SfaxSfaxTunisia
  2. 2.Laboratoire de Pathologie, CHU Habib BourguibaSfaxTunisia
  3. 3.Service de Biologie CliniqueCNSSSfaxTunisia

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