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Endocrine

, Volume 37, Issue 1, pp 129–134 | Cite as

Age, body mass index, and serum level of DHEA-S can predict glucocorticoid receptor function in women with polycystic ovary syndrome

  • Djuro MacutEmail author
  • Danijela Vojnović Milutinović
  • Ivana Božić
  • Gordana Matić
  • Jelena Brkljačić
  • Dimitrios Panidis
  • Milan Petakov
  • Nikolaos Spanos
  • Jelica Bjekić
  • Olivera Stanojlović
  • Anđela Petrović Milinković
  • Zoran Radojičić
  • Svetozar Damjanović
Original Article

Abstract

Glucocorticoid receptor (GR) transduces the glucocorticoid (GC) signal that could lead to metabolic derangements depending on the tissue responsiveness to GC. We aimed to investigate possible causative relation of the GR functional properties in peripheral blood mononuclear cells of women with polycystic ovary syndrome (PCOS), with their clinical and biochemical characteristics. Thirty women with PCOS [mean age: 26.5 ± 5.1 years, mean body mass index (BMI) 24.5 ± 5 kg/m2], and thirty respective controls were analyzed for the number of GR sites per cell (B max), apparent equilibrium dissociation constant (K d), and binding potency (GR potency). A strong association between B max and K d (r = 0.70, P < 0.0001), and GR potency with age (r = 0.49, P = 0.009) was observed in PCOS women. The multiple regression analyses within the PCOS group revealed that independent predictors for K d were BMI, total cholesterol, and dehydroepiandrosterone-sulfate (DHEA-S) (r = 0.58, P = 0.038), while for GR potency (r = 0.687, P = 0.013) were age, BMI, DHEA-S, and basal cortisol concentration. The results suggest that PCOS pathophysiology may be related to alterations of a cross stalk between glucocorticoid signaling, age, and metabolic parameters. These findings should be further explored in studies on the role of GR in PCOS-related metabolic derangements.

Keywords

PCOS Glucocorticoid receptor Glucocorticoid-receptor-binding characteristics DHEA-S 

Notes

Acknowledgments

This work was supported by the Ministry of Science of Serbia (Grant number 143003).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Djuro Macut
    • 1
    Email author
  • Danijela Vojnović Milutinović
    • 2
  • Ivana Božić
    • 1
  • Gordana Matić
    • 2
  • Jelena Brkljačić
    • 2
  • Dimitrios Panidis
    • 3
  • Milan Petakov
    • 1
  • Nikolaos Spanos
    • 3
  • Jelica Bjekić
    • 4
  • Olivera Stanojlović
    • 5
  • Anđela Petrović Milinković
    • 6
  • Zoran Radojičić
    • 7
  • Svetozar Damjanović
    • 1
  1. 1.Institute of Endocrinology, Diabetes and Metabolic DiseasesClinical Center of SerbiaBelgradeSerbia
  2. 2.Department of BiochemistryInstitute for Biological Research “Siniša Stanković”BelgradeSerbia
  3. 3.Division of Endocrinology and Human Reproduction, 2nd Department of Obstetrics and GynecologyAristotle University of ThessalonikiThessalonikiGreece
  4. 4.Department of EndocrinologyCHC Bežanijska kosaBelgradeSerbia
  5. 5.Institute of Physiology, School of MedicineUniversity of BelgradeBelgradeSerbia
  6. 6.Institute for Student’s HealthUniversity of BelgradeBelgradeSerbia
  7. 7.Faculty of Organizational Sciences, Institute for StatisticsUniversity of BelgradeBelgradeSerbia

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