Endocrine

, Volume 35, Issue 1, pp 112–117

Ghrelin stimulates proliferation of human osteoblastic TE85 cells via NO/cGMP signaling pathway

  • Deng-Hu Wang
  • Yun-Sheng Hu
  • Jun-Jie Du
  • Yun-Yu Hu
  • Wei-De Zhong
  • Wei-Jun Qin
Original Paper

DOI: 10.1007/s12020-008-9117-3

Cite this article as:
Wang, DH., Hu, YS., Du, JJ. et al. Endocr (2009) 35: 112. doi:10.1007/s12020-008-9117-3

Abstract

Ghrelin regulates bone formation and osteoblast proliferation, but the detailed signaling pathway for its action on osteoblasts remains unclear. In human osteoblastic TE85 cells, we observed the effects and intracellular signaling pathway of ghrelin on cell proliferation using BrdU incorporation method. Ghrelin, at 10−10–10−8 M concentration, significantly increased BrdU incorporation into TE85 cells. The action of ghrelin was inhibited by d-Lys3-GHRP-6, a selective antagonist of GHS-R. Nitric oxide (NO) scavenger hemoglobin and the NO synthase inhibitor NAME eliminated the stimulatory action of ghrelin on proliferation, while NO donor SNAP and NO synthase substrate L-AME stimulated proliferation of osteoblastic TE85 cells. The cGMP analogue, 8-Br-cGMP, stimulated TE85 cell proliferation, and ghrelin did not enhance proliferation in the presence of 8-Br-cGMP. Inhibition of cGMP production by the guanylate cyclase inhibitor prevented ghrelin-induced osteoblastic TE85 cell proliferation. In conclusion, ghrelin stimulates proliferation of human osteoblastic TE85 cells via intracellular NO/cGMP signaling pathway.

Keywords

Ghrelin Osteoblast Nitric oxide cGMP Proliferation 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Deng-Hu Wang
    • 1
  • Yun-Sheng Hu
    • 2
  • Jun-Jie Du
    • 1
  • Yun-Yu Hu
    • 1
  • Wei-De Zhong
    • 3
  • Wei-Jun Qin
    • 4
  1. 1.Department of Orthopedics, Xijing Hospital Fourth Military Medical UniversityXi’anChina
  2. 2.Department of Orthopedics, Tangdu HospitalFourth Military Medical UniversityXi’anChina
  3. 3.Research Center for Tumor, Guangzhou First Municipal People’s HospitalAffiliated Guangzhou Medical CollegeGuangzhouChina
  4. 4.State Key Laboratory of Cancer Biology, Xijing HospitalFourth Military Medical UniversityXi’anChina

Personalised recommendations