, Volume 34, Issue 1–3, pp 23–28

Evaluation of the association between retinal binding protein 4 polymorphisms and type 2 diabetes in Chinese by DHPLC

Original paper


Serum retinal binding protein 4 (RBP4) was recently described as a new liver- and adipocyte-derived signal that may contribute to Type 2 diabetes mellitus (T2DM) and insulin resistance. The aim of this study was to test whether the RBP4 gene could be used as a genetic marker to predict the development of T2DM amongst the Chinese population of Han. For this study, a normal control group of 115 healthy subjects and an experimental group of 107 patients with T2DM were examined. A combined method of denaturing high-performance liquid chromatography (DHPLC) and sequencing was applied to the detection of the RBP4 gene variants. Two SNPs, rs17484721 and rs36035572, were analyzed. Phenotypes and biochemical indicators related to the metabolism of glucose and lipid were measured. We found that there are significant differences between the control group and the patients group in terms of their respective distributions of genotype and allele frequency. The TG levels of the TT and II genotype was significantly higher than that of the TC + CC and ID + DD, respectively, in both patient group and control group. These findings suggest that the variations in the RBP4 gene may be associated with T2DM and serum triglyeride levels in the Han Chinese.


Retinal binding protein 4 Single nucleotide polymorphism Denaturing high-performance liquid chromatography Type 2 diabetes Mellitus Linkage disequilibrium 


  1. 1.
    Electronic version of Diabetes Atlas. Executive summary, 2nd ed. International Diabetes Federation. (2003). http://www.eatlas.idf.org/webdata/docs/Atlas%202003-Summary.pdf
  2. 2.
    M. Stumvoll, B.J. Goldstein, T.W. van Haeften, Lancet 365, 1333–1346 (2005)CrossRefPubMedGoogle Scholar
  3. 3.
    S.K. Das, S.C. Elbein, Cell Sci. Rev. 2, 1–32 (2006)Google Scholar
  4. 4.
    L. Hutley, J.B. Prins, Am. J. Med. Sci. 330, 280–289 (2005)CrossRefPubMedGoogle Scholar
  5. 5.
    Q. Yang, T.E. Graham, N. Mody, F. Preitner, O.D. Peroni, J.M. Zabolotny, K. Kotani, L. Quadro, B.B. Kahn, Nature 436, 356–362 (2005)CrossRefPubMedGoogle Scholar
  6. 6.
    Y. Tamori, H. Sakaue, M. Kasuga, Nat. Med. 12(1), 30–31 (2006)CrossRefPubMedGoogle Scholar
  7. 7.
    Y.M. Cho, B.S. Youn, H. Lee, N. Lee, S.S. Min, S.H. Kwak, H.K. Lee, K.S. Park, Diabetes Care 29, 2457–2461 (2006)CrossRefPubMedGoogle Scholar
  8. 8.
    M. Broch, J. Vendrell, W. Ricart, C. Richart, J.M. Fernandez Real, Diabetes Care 30, 1802–1806 (2007)CrossRefPubMedGoogle Scholar
  9. 9.
    T.E. Graham, Q. Yang, M. Bluher, A. Hammarstedt, T.P. Ciaraldi, R.R. Henry, C.J. Wason, A. Oberbach, P.A. Jansson, U. Smith, B.B.N. Kahn, Engl. J. Med. 354(24), 2552–2563 (2006)CrossRefGoogle Scholar
  10. 10.
    Q. Qi, Z. Yu, X. Ye, F. Zhao, P. Huang, F.B. H u, O.H. Franco, J. Wang, H. Li, Y. Liu, Lin X. Elevated, J. Clin. Endocrinol. Metab. 92(12), 4827–4834 (2007)CrossRefPubMedGoogle Scholar
  11. 11.
    A. Ost, A. Danielsson, M. Lidén, FASEB J. 21(13), 3696–3704 (2007)CrossRefPubMedGoogle Scholar
  12. 12.
    K.S. Polonsky, N. Engl. J. Med. 354, 2596–2598 (2006)CrossRefPubMedGoogle Scholar
  13. 13.
    J.B. Meigs, C.I. Panhuysen, R.H. Myers, P.W. Wilson, L.A. Cupples, Diabetes 51, 833–840 (2002)CrossRefPubMedGoogle Scholar
  14. 14.
    R. Duggirala, J. Blangero, L. Almasy, T.D. Dyer, K.L. Williams, R.J. Leach, P. O’Connell, M.P. Stern(1999). Am. J. Hum. Genet. 64:1127–1140.Google Scholar
  15. 15.
    R.L. Craig, W.S. Chu, S.C. Elbein, Mol. Genet. Metab. 90(3), 338–344 (2007)CrossRefPubMedGoogle Scholar
  16. 16.
    L. Munkhtulga, K. Nakayama, N. Utsumi, Y. Yanagisawa, T. Gotoh, T. Omi, M. Kumada, B. Erdenebulgan, K. Zolzaya, T. Lkhagvasuren, S. Iwamoto, Hum. Genet. 120(6), 879–888 (2007)CrossRefPubMedGoogle Scholar
  17. 17.
    P. Zimmet, Diabetes Care 2, 144–153 (1979)CrossRefPubMedGoogle Scholar
  18. 18.
    A.K. Diehl, M.P. Stern, Adv. Intern. Med. 34, 73–96 (1989)PubMedGoogle Scholar
  19. 19.
    P.M. McKeigue, B. Shah, M.G. Marmot, Lancet 337, 382–386 (1991)CrossRefPubMedGoogle Scholar
  20. 20.
    M.A. Banerji, H.E. Lebovitz, Diabetes 38, 784–792 (1989)CrossRefPubMedGoogle Scholar
  21. 21.
    P. Arner, T. Pollare, H. Lithell, Diabetologia 34, 483–487 (1991)CrossRefPubMedGoogle Scholar
  22. 22.
    A. Taniguchi, Y. Nakai, M. Fukushima, H. Kawamura, H. Imura, I. Nagata, K. Tokuyama, Diabetes 41, 1540–1546 (1992)CrossRefPubMedGoogle Scholar
  23. 23.
    A. Hirasawa, K. Tsumaya, T. Awaji, S. Katsuma, T. Adachi, M. Yamada, Y. Sugimoto, S. Miyazaki, G. Tsujimoto, Nat. Med. 11(1), 90–94 (2005)CrossRefPubMedGoogle Scholar
  24. 24.
    R. Perfetti, P. Merkel, Eur. J. Endocrinol. 143, 717–725 (2000)CrossRefPubMedGoogle Scholar
  25. 25.
    M. Dong, Y.H. Gong , L. Wang, Hereditas (Bejing) 25(2), 205–207 (2003)Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.People’s Hospital of Gansu ProvinceLanzhou CityChina
  2. 2.Affiliated Hospital of North China Coal Medical CollegeTangshan CityChina
  3. 3.First People’s Hospital of Lianyungang CityLianyungangChina
  4. 4.School of Basic Medical SciencesLanzhou UniversityLanzhou CityChina
  5. 5.School of Clinical MedicineLanzhou UniversityLanzhouChina

Personalised recommendations