Endocrine

, Volume 33, Issue 1, pp 40–44 | Cite as

In vitro assays to test the interference of anti-thyroglobulin antibodies on thyroglobulin measurement

  • Deolinda Madureira
  • Susana Prazeres
  • Márcia São Pedro
  • Teresa Pereira
  • Ana Paula Font
  • Maria João Bugalho
Original Paper

Abstract

Objective To assess the interference of anti-thyroglobulin antibodies (TgAb) on serum thyroglobulin (Tg) measurement by in vitro experiments. Design Re-evaluation of Tg concentration after dilution with different TgAb-positive sera. On a first step, dilutions of the same Tg with different TgAb sera were performed and on a second step, different Tgs were diluted with the same TgAb serum. Methods Tg measurements were performed using an immunometric (IMA) chemiluminescence assay. TgAb measurements were performed using two methods: immunoflurimetric assay (UNICAP 100) and IMA chemiluminescent assay (IMMULITE 2000). Results Dilution of a known concentration of Tg with different TgAb-positive sera resulted in a variation of the final concentration of Tg ranging from −24 to −79%. A weak correlation was observed between the TgAb concentration and the percentage of the Tg deviation. Dilution of different Tgs with the same TgAb-positive serum illustrated how the same TgAb positive serum may determine a high interference or a neutral effect. Conclusions Present results suggest that the interference on Tg measurement observed in the presence of TgAb may result not only from the anti-thyroglobulin antibodies, but also from the thyroglobulin itself.

Keywords

Thyroid Biomarker Thyroglobulin Antibodies 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Deolinda Madureira
    • 1
  • Susana Prazeres
    • 1
  • Márcia São Pedro
    • 1
  • Teresa Pereira
    • 1
  • Ana Paula Font
    • 1
  • Maria João Bugalho
    • 2
  1. 1.Laboratório de EndocrinologiaInstituto Português de Oncologia de Lisboa, Francisco Gentil E.P.E.LisbonPortugal
  2. 2.Serviço de EndocrinologiaInstituto Português de Oncologia de Lisboa, Francisco Gentil E.P.E.LisbonPortugal

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