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Endocrine

, Volume 31, Issue 1, pp 72–81 | Cite as

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

  • Nader S. Shenouda
  • Mary S. Sakla
  • Leslie G. Newton
  • Cynthia Besch-Williford
  • Norman M. Greenberg
  • Ruth S. MacDonald
  • Dennis B. LubahnEmail author
Original Paper

Abstract

Background

Prostate cancer is an important public health problem. It is an excellent candidate disease for chemo-prevention because prostate cancer is typically slow growing and is usually diagnosed in elderly males. Pygeum africanum (Prunus africana or Rosaceae) is an African prune (plum) tree found in tropical Africa. An extract from the bark of Pygeum africanum has been used in Europe as a prevention and treatment of prostate disorders including benign prostatic hypertrophy (BPH). More recently in the USA, the phytotherapeutic preparations of Pygeum africanum and Saw palmetto have been marketed for prostate health including prostate cancer prevention and treatment.

Methods

The anti-cancer potential of Pygeum africanum has been tested both in vitro (PC-3 and LNCaP cells) and in␣vivo (TRAMP mouse model).

Results

In tissue culture, ethanolic extracts (30%) of Pygeum africanum inhibited the growth of PC-3 and LNCaP cells; induced apoptosis and altered cell kinetics; down regulated ERα and PKC-α protein, and demonstrated good binding ability to both mouse uterine estrogen receptors and LNCaP human androgen receptors. TRAMP mice fed Pygeum africanum showed a significant reduction (P = 0.034) in prostate cancer incidence (35%) compared to casein fed mice (62.5%).

Conclusion

Pygeum africanum, which is widely used in Europe and USA for treatment of BPH, has a significant role in regulation of prostate cancer both in␣vitro and in␣vivo and therefore may be a useful supplement for people at high risk for developing prostate cancer.

Keywords

African herb LNCaP PC-3 TRAMP mice 

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Notes

Acknowledgments

We would like to thank Dr. Wendy Applequest, Missouri Botanical Gardens for her kind assistance in obtaining the Pygeum africanum extract. We appreciate the help of Dr. George Rottinghaus, Department of Analytical chemistry, University of Missouri-Columbia in making the ethanol extract of Pygeum africanum. This work was supported by the Missouri University Center for Phytonutrient and Phytochemical Studies (MUCCPS), NIH Grant # P01-ES510535 and NIH grant # R01AT002978.

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Nader S. Shenouda
    • 1
  • Mary S. Sakla
    • 1
  • Leslie G. Newton
    • 1
  • Cynthia Besch-Williford
    • 2
  • Norman M. Greenberg
    • 5
  • Ruth S. MacDonald
    • 6
  • Dennis B. Lubahn
    • 1
    • 3
    • 4
    • 7
    Email author
  1. 1.Department of BiochemistryUniversity of Missouri-ColumbiaColumbiaUSA
  2. 2.Department of Veterinary PathobiologyUniversity of Missouri-ColumbiaColumbiaUSA
  3. 3.Department of Child HealthUniversity of Missouri-ColumbiaColumbiaUSA
  4. 4.Department of Animal SciencesUniversity of Missouri-ColumbiaColumbiaUSA
  5. 5.Fred Hutchinson Cancer Research CenterSeattleUSA
  6. 6.Department of Food Science and Human NutritionIowa State UniversityAmesUSA
  7. 7.MU Center for Phytonutrient and Phytochemical StudiesColumbiaUSA

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