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NeuroMolecular Medicine

, Volume 18, Issue 3, pp 334–346 | Cite as

Neuroprotective Effects of Germinated Brown Rice in Rotenone-Induced Parkinson’s-Like Disease Rats

  • Supin Chompoopong
  • Sunit Jarungjitaree
  • Tideeporn Punbanlaem
  • Thanaporn Rungruang
  • Sukumal Chongthammakun
  • Aikkarach Kettawan
  • Thongchai Taechowisan
Original Paper

Abstract

The effects of germinated brown rice (GBR) on the motor deficits and the dopaminergic (DA) cell death were investigated in Parkinson’s-like disease (PD) rats. Reactive oxidative species generated by chronic subcutaneous injection of rotenone (RT) lead to neuronal apoptosis particularly in the nigrostriatal DA system and produce many features of PD, bradykinesis, postural instability and rigidity. In this study, 4-phenylbutyric acid (4-PBA), previously reported to inhibit RT-induced DA cell death, was used as the positive control. Results show that pretreatment with GBR as well as 4-PBA significantly enhanced the motor activity after RT injection, and GBR affected significantly in open field test, only in the ambulation but not the mobility duration, and ameliorated the time to orient down (t-turn) and total time to descend the pole (t-total) in pole test as compared to RT group, but significantly lowered both t-turn and t-total only in 4-PBA group. The percentage of apoptotic cells in brain measured by flow cytometry and the inflammatory effect measured by ELISA of TNF-α showed significant increase in RT group as compared to the control (CT) group at P < 0.05. Apoptotic cells in RT group (85.98 %) showed a significant (P < 0.05) increase versus CT group (17.50 %), and this effect was attenuated in GBR+RT group by decreasing apoptotic cells (79.32 %), whereas, increased viable cells (17.94 %) versus RT group (10.79 %). GBR in GBR + RT group could decrease TNF-α both in the serum and in brain. In summary, GBR showed a neuroprotective effect in RT-induced PD rats, and it may be useful as a value-added functional food to prevent neurodegenerative disease or PD.

Keywords

Parkinson’s disease Rotenone Neurodegeneration Germinated brown rice 4-phenylbutyrate 

Notes

Acknowledgments

The authors gratefully acknowledge Miss Kanittar Srisook, Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, for helping in the histopathological examination. We are very grateful for the generous providing of GBR from Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no personal or professional conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Supin Chompoopong
    • 1
  • Sunit Jarungjitaree
    • 1
  • Tideeporn Punbanlaem
    • 1
  • Thanaporn Rungruang
    • 1
  • Sukumal Chongthammakun
    • 2
  • Aikkarach Kettawan
    • 3
  • Thongchai Taechowisan
    • 4
  1. 1.Department of Anatomy, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand
  2. 2.Department of Anatomy, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Institute of NutritionMahidol UniversitySalaya, NakhonpathomThailand
  4. 4.Department of Microbiology, Faculty of ScienceSilpakorn UniversityNakhonpathomThailand

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