Kinesin Light Chain 1 Gene Haplotypes in Three Conformational Diseases
- 143 Downloads
A functional intracellular transport system is essential to maintain cell shape and function especially in elongated cells, e.g. neurons and lens fibre cells. Impaired intracellular transport has been suggested as a common pathological mechanism for age-related diseases characterised by protein aggregation. Here, we hypothesise that common genetic variation in the transport protein kinesin may influence the risk of Parkinson’s disease (PD), Alzheimer’s disease (AD) and age-related cataract. This case–control study involves a PD material (165 cases and 190 controls), an AD material (653 cases and 845 controls) and a cataract material (495 cases and 183 controls). Genetic variation in the kinesin light chain 1-encoding gene (KLC1) was tagged by six tag single nucleotide polymorphisms (SNPs). Single SNPs and haplotypes were analysed for associations with disease risk, age parameters, mini-mental state examination scores and cerebrospinal fluid biomarkers for AD using logistic or linear regression. Genetic variation in KLC1 did not influence risk of PD. Weak associations with risk of AD were seen for rs8007903 and rs3212079 (P c = 0.04 and P c = 0.02, respectively). Two SNPs (rs8007903 and rs8702) influenced risk of cataract (P c = 0.0007 and P c = 0.04, respectively). However, the allele of rs8007903 that caused increased risk of AD caused reduced risk of cataract, speaking against a common functional effect of this particular SNP in the two diseases. Haplotype analyses did not add significantly to the associations found in the single SNP analyses. Altogether, these results do not convincingly support KLC1 as a major susceptibility gene in any of the studied diseases, although there is a small effect of KLC1 in relation to cataract.
KeywordsParkinson’s disease Alzheimer’s disease Cataract Kinesin KLC1 SNP Haplotype
The authors are grateful for the excellent technical assistance of Mrs Mona Seibt Palmér. This work was supported by grants from the Swedish Research Council (projects 2006-6227, 2006-2740, 2006-3505 and 09946), the Alzheimer’s Association (NIRG-08-90356), cNEUPRO, the Royal Swedish Academy of Sciences, the Sahlgrenska University Hospital, the Göteborg Medical Society, the Swedish Medical Society, Swedish Brain Power, Stiftelsen Gamla Tjänarinnor, Ragnar och Einar Lundströms minne, Adlerbertska forskningsstiftelsen, Pfannenstills stiftelse, Wilhelm och Martina Lundgrens stiftelse, Gun och Bertil Stohnes stiftelse, Åhlén-stiftelsen, Kronprinsessan Margaretas Arbetsnämnd för Synskadade, Stiftelsen Hjalmar Svenssons Forskningsfond and Alzheimer Foundation, Sweden.
- Bergman, O., Hakansson, A. et al. (2008). PITX3 polymorphism is associated with early onset Parkinson’s disease. Neurobiology of Aging doi: 10.1016/j.neurobiolaging.2008.03.008.
- Bertram, L., McQueen, M. B. et al. (2007). Systematic meta-analyses of Alzheimer disease genetic association studies: The AlzGene database. Nature Genetics, 39(1), 17–23. Accessed on Sept. 24, 2009.Google Scholar
- Lill, C. M., Bagade, S. et al. The PDGene Database. Alzheimer Research Forum. Accessed October 7, 2009. Available at: http://www.pdgene.org/.
- Szolnoki, Z., Kondacs, A., et al. (2007b). A genetic variant in cytoskeleton motors amplifies susceptibility to leukoaraiosis in hypertensive smokers: Gene-environmental interactions behind vascular white matter demyelinization. Journal of Molecular Neuroscience, 33(2), 173–179.CrossRefPubMedGoogle Scholar