Clinical Reviews in Allergy & Immunology

, Volume 34, Issue 3, pp 321–325 | Cite as

Anti-α-Actinin Antibodies Cross-react with Anti-ssDNA Antibodies in Active Autoimmune Hepatitis

  • Yves Renaudineau
  • Geogios N. Dalekos
  • Paul Guéguen
  • Kalliopi Zachou
  • Pierre Youinou


Recently, we have observed that the association of anti-F-actin autoantibodies (Abs) with anti-α-actinin Abs (double reactivity) was correlated with liver disease activity in autoimmune hepatitis type 1 (AIH-1; Guéguen et al., J Clin Immunol 26:495–505, 2006). As anti-α-actinin Abs are also associated with anti-single-stranded (ss) DNA in 16 of 50 (32%) AIH-1 patients, compared with 16 of 401 (4.0%) liver disease control patients, we sought to characterize these Abs in AIH-1. When associated, anti-ssDNA Abs cross-react with anti-α-actinin Abs, but neither with anti-double-stranded DNA Abs nor with anti-F-actin Abs. Furthermore, anti-α-actinin Abs are associated with high-avidity anti-ssDNA Abs in AIH-1. In addition, double reactivity against α-actinin and ssDNA was correlated with clinical and histological activity of the disease and untreated AIH-1 patients.


Autoimmune hepatitis type 1 Anti-DNA Anti-α-actinin 



Many thanks are due to Cindy Séné for secretarial assistance. This work has been supported in part by a grant (#2466) of the Research Committee, University of Thessaly.


  1. 1.
    Zachou K, Rigopoulou E, Dalekos GN (2004) Autoantibodies and autoantigens in autoimmune hepatitis: important tools in clinical practice and to study pathogenesis of the disease. J Autoimmun Dis 1:2CrossRefGoogle Scholar
  2. 2.
    Guéguen P, Dalekos G, Nousbaum JB et al (2006) Double reactivity against actin and alpha-actinin defines a severe form of autoimmune hepatitis type 1. J Clin Immunol 26:495–505PubMedCrossRefGoogle Scholar
  3. 3.
    Mostoslavsky G, Fischel R, Yachimovich N et al (2001) Lupus anti-DNA autoantibodies cross-react with a glomerular structural protein: a case for tissue injury by molecular mimicry. Eur J Immunol 31:1221–1227PubMedCrossRefGoogle Scholar
  4. 4.
    Deocharan B, Qing X, Lichauco J, Putterman C (2002) Alpha-actinin is a cross-reactive renal target for pathogenic anti-DNA antibodies. J Immunol 168:3072–3078PubMedGoogle Scholar
  5. 5.
    Alvarez F, Berg PA, Bianchi FB et al (1999) International autoimmune hepatitis group report: review of criteria for diagnosis of autoimmune hepatitis. J Hepatol 31:929–938PubMedCrossRefGoogle Scholar
  6. 6.
    Croquefer S, Renaudineau Y, Jousse S et al (2005) The anti-alpha-actinin test completes anti-DNA determination in systemic lupus erythematosus. Ann NY Acad Sci 1050:170–175PubMedCrossRefGoogle Scholar
  7. 7.
    Renaudineau Y, Croquefer S, Jousse S et al (2006) Association of alpha-actinin-binding anti-double-stranded DNA antibodies with lupus nephritis. Arthritis Rheum 54:2523–2532PubMedCrossRefGoogle Scholar
  8. 8.
    Mason LJ, Ravirajan CT, Rahman A, Putterman C, Isenberg DA (2004) Is alpha-actinin a target for pathogenic anti-DNA antibodies in lupus nephritis? Arthritis Rheum 50:866–870PubMedCrossRefGoogle Scholar
  9. 9.
    Leibovitch L, George J, Levi Y, Bakimer R, Shoenfeld Y (1995) Anti-actin antibodies in sera from patients with autoimmune liver diseases and patients with carcinomas by ELISA. Immunol Lett 48:129–132PubMedCrossRefGoogle Scholar
  10. 10.
    Dalekos GN, Makri E, Loges S et al (2002) Increased incidence of anti-LKM autoantibodies in a consecutive cohort of hepatitis C patients from central Greece. Eur J Gastroenterol Hepatol 14:35–42PubMedCrossRefGoogle Scholar
  11. 11.
    Dalekos GN, Wedemeyer H, Obermayer-straub P et al (1999) Epitope mapping of cytochrome P4502D6 autoantigen in patients with chronic hepatitis C during alpha-interferon treatment. J Hepatol 30:366–375PubMedCrossRefGoogle Scholar
  12. 12.
    Renaudineau Y, Revelen R, Bordron A, Mottier D, Youinou P, Le corre R (1998) Two populations of endothelial cell antibodies cross-react with heparin. Lupus 7:86–94PubMedCrossRefGoogle Scholar
  13. 13.
    Moritoki Y, Lian ZX, Ohsugi Y, Ueno Y, Gershwin ME (2006) B cells and autoimmune liver diseases. Autoimmun Rev 5:449–457PubMedCrossRefGoogle Scholar
  14. 14.
    Youinou P (2007) B cell conducts the lymphocyte orchestra. J Autoimmun 28:143–151PubMedCrossRefGoogle Scholar
  15. 15.
    Christen U, Holdener M, Hintermann E (2007) Animal models for autoimmune hepatitis. Autoimmun Rev 6:306–311PubMedCrossRefGoogle Scholar
  16. 16.
    Renaudineau Y, Deocharan B, Jousse S, Renaudineau E, Putterman C, Youinou P (2007) Anti-alpha-actinin antibodies: a new marker of lupus nephritis. Autoimmun Rev 6:464–468PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Yves Renaudineau
    • 1
  • Geogios N. Dalekos
    • 2
  • Paul Guéguen
    • 1
  • Kalliopi Zachou
    • 2
  • Pierre Youinou
    • 1
    • 3
  1. 1.Brest University Medical School HospitalBrest CedexFrance
  2. 2.Thessaly University Medical SchoolLarissaGreece
  3. 3.Laboratory of ImmunologyBrest University Medical School HospitalBrest CedexFrance

Personalised recommendations