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Clinical Reviews in Allergy & Immunology

, Volume 34, Issue 1, pp 4–10 | Cite as

Antibodies to Citrullinated Vimentin are a Specific and Sensitive Marker for the Diagnosis of Rheumatoid Arthritis

  • Hannah Poulsom
  • Peter J. CharlesEmail author
Article

Abstract

Background

The last 5 years have seen the emergence and establishment of antibodies to citrullinated antigens as the diagnostic marker for rheumatoid arthritis (RA). Initially, these were detected using a synthetic peptide, which has undergone a number of modifications to give a diagnostic test with a sensitivity of 65–80% and a specificity of >95%. Antibodies to citrullinated vimentin were first described in 1994 as a highly specific marker for RA (anti-Sa). However, no easily performed assay for these antibodies has been available.

Methods

We have examined the use of a ELISA-based assay with a mutated citrullinated vimentin (MCV) antigen (Orgentec, Mainz, Germany) to assess the diagnostic and prognostic utility of this antibody in RA.

Results

Antibodies to MCV were detected in the sera of 74% RA patients (specificity 96%), 2% systemic lupus erythematosus, 14% Sjögren’s syndrome, and 2% scleroderma. Anti-MCV was not detected in sera from healthy blood donors. There was no difference in the frequency of antibodies detected in RA patients with early (<2 years) or chronic (>2 years) disease. There was no significant variation in anti-MCV antibody concentrations in early RA patients over a 52-week period. No significant change was observed with time between the two treatment groups of methotrexate alone or methotrexate plus infliximab.

Conclusions

Antibodies to MCV are a specific and sensitive marker for the diagnosis of RA.

Keyword

Mutated citrullinated vimentin Anti-MCV Autoantibodies Rheumatoid arthritis 

References

  1. 1.
    Huizinga TWJ, Landewé RBM (2005) Nat Clin Pract Rheum 1(1):2–3CrossRefGoogle Scholar
  2. 2.
    Arnett FC, Edworthy SM, Bloch DA et al (1988) Arthritis Rheum 31:315–324PubMedCrossRefGoogle Scholar
  3. 3.
    Cruyssen BV, Cantaert T, Nogueira L et al (2006) Arthritis Res Ther 8:R122CrossRefGoogle Scholar
  4. 4.
    Kinloch A, Tatzer V, Wait R et al (2005) Arthritis Res Ther 7:R1421–R1429PubMedCrossRefGoogle Scholar
  5. 5.
    Vossenaar ER, Radstake TR, Van Der Heijden A et al (2004) Ann Rheum Dis 63:373–381PubMedCrossRefGoogle Scholar
  6. 6.
    Tak PP, Smeets TJ, Daha MR et al (1997) Arthritis Rheum 40:217–225PubMedCrossRefGoogle Scholar
  7. 7.
    Zendman AJW, van Venrooij WJ, Pruijn GJM (2006) Rheumatology 45:20–25PubMedCrossRefGoogle Scholar
  8. 8.
    Tarcsa E, Marekov LN, Mei G et al (1996) J Biol Chem 271:30709–30716PubMedCrossRefGoogle Scholar
  9. 9.
    Lundberg K, Nijenhuis S, Vossenaar E et al (2004) Arthritis Res Ther 6(Suppl 1):18 (Abstract)CrossRefGoogle Scholar
  10. 10.
    Hill JA, Southwood S, Sette A et al (2003) J Immunol 171:538–541PubMedGoogle Scholar
  11. 11.
    van Gaalen F, Ioan-Facsinay A, Huizinga TWJ et al (2005) J Immunol 175:5575–5580PubMedGoogle Scholar
  12. 12.
    Masson-Bessiere C, Sebbag M, Durieux JJ et al (2000) Clin Exp Immunol 119:544–552PubMedCrossRefGoogle Scholar
  13. 13.
    Makrygiannakis D, af Klint E, Lundberg IE et al (2006) Ann Rheum Dis 65:1219–1222PubMedCrossRefGoogle Scholar
  14. 14.
    Young BJ, Mallya RK, Leslie RD et al (1979) Br Med J 14:97–99CrossRefGoogle Scholar
  15. 15.
    Simon M, Girbal E, Sebbag M et al (1993) J Clin Invest 92:1387–1393PubMedGoogle Scholar
  16. 16.
    Sebbag M, Simon M, Vincent C et al (1995) J Clin Invest 95:2672–2679PubMedGoogle Scholar
  17. 17.
    Girbal-Neuhauser E, Durieux JJ, Arnaud M et al (1999) J Immunol 162:585–594PubMedGoogle Scholar
  18. 18.
    van Venrooij WJ, Pruijn GJM (2000) Arthritis Res 2:249–251PubMedCrossRefGoogle Scholar
  19. 19.
    Nielen MMJ, van der Horst AR, van Schaardenburg D et al (2005) Ann Rheum Dis 64:1199–1204PubMedCrossRefGoogle Scholar
  20. 20.
    Schellekens GA, de Jong BA, van den Hoogen FH et al (1998) J Clin Invest 101:273–281PubMedCrossRefGoogle Scholar
  21. 21.
    Quinn MA, Gough AK, Green MJ et al (2006) Rheumatology 45(4):478–480PubMedCrossRefGoogle Scholar
  22. 22.
    van Gaalen FA, Visser H, Huizinga TWJ (2005) Ann Rheum Dis 64:1510–1512PubMedCrossRefGoogle Scholar
  23. 23.
    Takasaki Y, Yamanaka K, Takasaki C et al (2004) Mod Rheumatol 14(5):367–375PubMedCrossRefGoogle Scholar
  24. 24.
    Vannini A, Cheung K, Fusconi M et al (2006) Ann Rheum Dis (published online http://ard.bmj.com/cgi/content/abstract/ard.2006.058933v1)
  25. 25.
    Rantapaa-Dahlqvist S, de Jong BA, Berglin E et al (2003) Arthritis Rheum 48(10):2701–2705CrossRefGoogle Scholar
  26. 26.
    Van Gaalen FA, Linn-Rasker SP, van Venrooij WJ et al (2004) Arthritis Rheum 50(3):709–715PubMedCrossRefGoogle Scholar
  27. 27.
    Berglin E, Padyukov L, Sundin U et al (2004) Arthritis Res Ther 6:R303–R308PubMedCrossRefGoogle Scholar
  28. 28.
    van der Helm-van Mil AHM, Verpoort KN, Breedveld FC et al (2005) Arthritis Res Ther 7(5):R949–R958PubMedCrossRefGoogle Scholar
  29. 29.
    Berglin E, Johansson T, Sundin U et al (2006) Ann Rheum Dis 65(4):453–458PubMedCrossRefGoogle Scholar
  30. 30.
    Inanc N, Dalkilic E, Kamali S et al (2007) Clin Rheumatol 26(1):17–23PubMedCrossRefGoogle Scholar
  31. 31.
    Despres N, Boire G, Lopez-Longo FJ et al (1994) J Rheumatol 21(6):1027–1033PubMedGoogle Scholar
  32. 32.
    Menard HA, Lapointe E, Rochdi MD et al (2000) Arthritis Res 2:429–432PubMedCrossRefGoogle Scholar
  33. 33.
    Hueber W, Hassfeld W, Smolen JS et al (1999) Rheumatology 38:155–159PubMedCrossRefGoogle Scholar
  34. 34.
    Hayem G, De Bandt M, Palazzo E et al (1999) J Rheumatol 26:7–13PubMedGoogle Scholar
  35. 35.
    Vossenaar ER, Despres N, Lapointe E et al (2004) Arthritis Res Ther 6(2):R142–R150PubMedCrossRefGoogle Scholar
  36. 36.
    Boire G, Cossette P, de Brum-Fernandes AJ et al (2005) Arthritis Res Ther 7:R592–R603PubMedCrossRefGoogle Scholar
  37. 37.
    Bang H, Lûthke K, Gauliard A et al (2006) Ann Rheum DIs 65(Suppl II):144 (Abstract)Google Scholar
  38. 38.
    Lakos G, Soos L, Szabo Z et al (2006) Abstract THU0118, EULARGoogle Scholar
  39. 39.
    Klotz W, Dejaco C, Larcher H et al (2006) Abstract FRI0050, EULARGoogle Scholar
  40. 40.
    Brickmann KP, Yazdani-Biuki BJ, Graninger M et al (2006) Abstract SAT0431, EULARGoogle Scholar
  41. 41.
    Dejaco C, Klotz W, Larcher H et al (2006) Arthritis Res Ther 8:R119–R124PubMedCrossRefGoogle Scholar
  42. 42.
    Hassfeld WG, Vinje O, König K et al (2006) Abstract FR10513, EULARGoogle Scholar
  43. 43.
    Brickmann K, Yazdani-Biuki BJ, Mûller T et al (2006) Abstract AB0492, EULARGoogle Scholar
  44. 44.
    Egerer K, Bang H, Gauliard A et al (2006) Poster presented at EULARGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  1. 1.Division of ImmunologyHammersmith Hospitals NHS TrustLondonUK
  2. 2.Kennedy Institute of RheumatologyImperial CollegeLondonUK
  3. 3.Division of ImmunologyCharing Cross HospitalLondonUK

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