Nongonadal tissues express luteinizing hormone-chorionic gonadotropin receptors (LHCG-R) which are essential for their growth during fetal development. Adult mesenchymal stem/stromal cells (MSCs) have been shown to express functional LHCG-R outside pregnancy conditions, making them susceptible to hCG stimulation. In the present study we tested the effect of hCG treatment on bone marrow (BM) derived adherent stem cells in vitro, isolated from a parous women, mother of male sons, in order to evaluate its effect on maternal MSCs and in the same time on fetal microchimeric stem cells (FMSCs), to better understand the outcomes of this safe and affordable treatment on cell proliferation and expression of pluripotency genes. Our study highlights the beneficial effects of hCG exposure on gene regulation in bone marrow adherent stem cells through the upregulation of pluripotency genes and selection of more primitive mesenchymal stem cells with a better differentiation potential. Validation of these effects on MSCs and FMSCs long after parturition in vivo represents a close perspective as it could set the premises of a new mobilization strategy for the stem cell transplantation procedures in the clinical setting.
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Conflict of Interests
All authors have reviewed and approved this version of the manuscript, and prudent care was taken to confirm the integrity of the work. No fragment of this article has been published or submitted elsewhere. There is no financial conflict of interest in the submission of this article.
The study involving human participants was approved by the ethics committee of the University of Medicine and Pharmacy “Iuliu Hatieganu” Cluj-Napoca, reg. no. 100/08.03.2017 and informed consent was obtained prior to tissue sample analysis.
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Cismaru, A.C., Soritau, O., Jurj, A.M. et al. Human Chorionic Gonadotropin Improves the Proliferation and Regenerative Potential of Bone Marrow Adherent Stem Cells and the Immune Tolerance of Fetal Microchimeric Stem Cells In Vitro. Stem Cell Rev and Rep (2020). https://doi.org/10.1007/s12015-020-09957-w
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