Stem Cell Reviews and Reports

, Volume 13, Issue 1, pp 139–148 | Cite as

A Regenerative Approach with Dermal Micrografts in the Treatment of Chronic Ulcers

  • Francesco De FrancescoEmail author
  • Antonio Graziano
  • Letizia Trovato
  • Gabriele Ceccarelli
  • Maurizio Romano
  • Marco Marcarelli
  • Gabriella Maria Cusella De Angelis
  • Umberto Cillo
  • Michele Riccio
  • Giuseppe Andrea Ferraro



The etiology of non-healing ulcers depends on both systemic and local factors. The introduction of advanced dressing, negative wound therapy and compression therapy have undoubtedly improved clinical outcomes. The principal aim of study was to demonstrate the efficacy of dermal micrografts in the treatment of ulcers with different etiologies. The second aim was to investigate in vitro the action of micrografts in the regenerative process.


The dermal micro-grafts were obtained from mechanical disaggregation of small pieces of skin tissue through a medical device called Rigeneracons.


We observed in vivo the ability of dermal autologous micrografts to improve the healing of venous, diabetic, pressure and post-traumatic ulcers after few week of treatment accomplished in general with a better quality of life for the patients. In vitro results showed that these micrografts express mesenchymal stem cells (MSCS) marker such as CD34, CD73, CD90 and CD105, and are able to form a viable and proliferative biocomplex with collagen sponge. Finally, the site of ulcers displayed a different expression of epidermal growth factors, insulin-like growth factors, platelet-derived growth factors and their receptors and tumor necrosis factor-β with respect to healthy skin samples.


We reported a good outcome for the treatment of chronic ulcers using dermal autologous micrografts. Finally, we suggest that the positivity to MSCs markers and the ability to interact with a scaffold can play a key role in their regenerative properties.


Wound healing Micrograft Mesenchymal stem cells Growth factors Ulcers 



We thank Prof. Giuseppina Caraglia B.A., English language expert and assistant for Department of Sciences and Environmental, Biological, Pharmaceutical Technologies, Caserta, Second University of Naples for providing excellent technical revision and support.

Compliance with Ethical Standards

Conflict of Interest

The authors A.G. and L.T. are members of Scientific Division of Human Brain Wave srl, the manufacturer of the Rigeneracons medical device used in this study. No funding. Other authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Francesco De Francesco
    • 1
    Email author
  • Antonio Graziano
    • 2
    • 3
  • Letizia Trovato
    • 2
  • Gabriele Ceccarelli
    • 4
    • 5
  • Maurizio Romano
    • 6
  • Marco Marcarelli
    • 7
  • Gabriella Maria Cusella De Angelis
    • 4
    • 5
  • Umberto Cillo
    • 6
  • Michele Riccio
    • 8
  • Giuseppe Andrea Ferraro
    • 1
  1. 1.Multidisciplinary Department of Medical-Surgical and Dental SpecialtiesSecond University of NaplesNaplesItaly
  2. 2.Human Brain Wave srlTorinoItaly
  3. 3.SHRO – Temple University of PhiladelphiaPhiladelphiaUSA
  4. 4.Department of Public Health, Experimental Medicine and ForensicsUniversity of PaviaPaviaItaly
  5. 5.C.I.T., Tissue Engineering CentreUniversity of PaviaPaviaItaly
  6. 6.Department of Surgery, Oncology and Gastroenterology, Hepatobiliary Surgery and Liver TransplantationPadua University HospitalPaduaItaly
  7. 7.Moncalieri Hospital, ASL To5 – U.C.S. Orthopedics and TraumatologyMoncalieri TOItaly
  8. 8.Department of Reconstructive Plastic Surgery-Hand SurgeryAOU “Ospedali Riuniti”AnconaItaly

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