Engineering Cell Fate for Tissue Regeneration by In Vivo Transdifferentiation
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Changes in cell identity occur in adult mammalian organisms but are rare and often linked to disease. Research in the last few decades has thrown light on how to manipulate cell fate, but the conversion of a particular cell type into another within a living organism (also termed in vivo transdifferentiation) has only been recently achieved in a limited number of tissues. Although the therapeutic promise of this strategy for tissue regeneration and repair is exciting, important efficacy and safety concerns will need to be addressed before it becomes a reality in the clinical practice. Here, we review the most relevant in vivo transdifferentiation studies in adult mammalian animal models, offering a critical assessment of this potentially powerful strategy for regenerative medicine.
Key wordsTransdifferentiation Reprogramming Cell fate Regeneration Tissue repair
Irene de Lázaro would like to thank Obra Social LaCaixa, University College London (UCL) and the University of Manchester for jointly funding this project.
Conflicts of interest
The authors indicate no potential conflicts of interest.
- 4.Weintraub, H., Tapscott, S. J., Davis, R. L., et al. (1989). Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. Proceedings of the National Academy of Sciences of the United States of America, 86(14), 5434–8.PubMedCentralCrossRefPubMedGoogle Scholar
- 38.Niu, W., T. Zang, D.K. Smith, et al. (2015). SOX2 Reprograms resident astrocytes into neural progenitors in the adult brain. Stem Cell Reports.Google Scholar
- 44.Vivien, C., Scerbo, P., Girardot, F., Le Blay, K., Demeneix, B. A., & Coen, L. (2012). Non-viral expression of mouse Oct4, Sox2, and Klf4 transcription factors efficiently reprograms tadpole muscle fibers in vivo. Journal of Biological Chemistry, 287(10), 7427–35.PubMedCentralCrossRefPubMedGoogle Scholar
- 46.Yilmazer, A., I. de Lazaro, C. Bussy, and K. Kostarelos. (2013). In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors. JoVE 17(82).Google Scholar