Stem Cell Reviews and Reports

, Volume 9, Issue 1, pp 80–92 | Cite as

Mesenchymal Stem Cell: Does it Work in an Experimental Model with Acute Respiratory Distress Syndrome?

  • Sema YilmazEmail author
  • Nihal Inandiklioglu
  • Dincer Yildizdas
  • Cansu Subasi
  • Arbil Acikalin
  • Yurdun Kuyucu
  • Ibrahim Bayram
  • Ali Topak
  • Atila Tanyeli
  • Gokhan Duruksu
  • Erdal KaraozEmail author


We hypothesized that bone marrow-derived mesenchymal stem cells (BM-MSCs) would have a possible role in the treatment of acute respiratory distress syndrome (ARDS). ARDS disease model was developed in Wistar albino male rats by intratracheal instillation of physiological saline solution. Anesthezied and tracheotomized rats (n = 8) with ARDS were pressure-controlled ventilated. Isolated and characterized rat (r-) BM-MSCs were labeled with GFP gene, and introduced in the lungs of the ARDS rat-model. After applying of MSCs, the life span of each rat was recorded. When rats died, their lung tissues were removed for histopathological examination. Also the tissue sections were analyzed for GFP labeled rBM-MSCs and stained for vimentin, CK19, proinflammatory (MPO, IL-1β, IL-6 and MIP-2) and anti-inflammatory [IL-1ra and prostaglandin E2 receptor (EP3)] cytokines. The histopathological signs of rat-model ARDS were similar to the acute phase of ARDS in humans. rBM-MSCs were observed to home in lung paranchyma. Although the infiltration of neutrophils slightly decreased in the interalveolar, peribronchial and perivascular area, a notable improvement was determined in the degree of hemorrhage, edema and hyaline membrane formation in rats treated with rBM-MSCs. Also decreased proinflammatory cytokines levels and increased the intensity of anti-inflammatory cytokines were established. Therefore MSCs could promote alveoar epithelial repair by mediating of cytokines from a proinflammatory to an anti-inflammatory response. As a novel therapeutic approach, mesenchymal stem cell treatment with intratracheal injection could be helpful in the management of critically ill patients with ARDS.


Acute respiratory distress syndrome Mesenchymal stem cell Cytokine Inflammation Critically ill 



This work was supported by the Cukurova University Research (TF2011BAP20) Fund. The authors thank Kezban Bostaner, Recep Mutlu and Gizem Turaç for their technical assistance. We also express our gratitude to Dr. Gökhan Duruksu for editing of this manuscript.

Conflict of Interest Statement

None of the authors have a financial relationship with a commercial entity that has an interest in the subject of this manuscript.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Sema Yilmaz
    • 1
    Email author
  • Nihal Inandiklioglu
    • 2
  • Dincer Yildizdas
    • 3
  • Cansu Subasi
    • 4
  • Arbil Acikalin
    • 5
  • Yurdun Kuyucu
    • 6
  • Ibrahim Bayram
    • 7
  • Ali Topak
    • 8
  • Atila Tanyeli
    • 7
  • Gokhan Duruksu
    • 9
  • Erdal Karaoz
    • 4
    Email author
  1. 1.Department of Pediatric Hematology/OncologyOndokuz Mayıs University Faculty of MedicineAtakumTurkey
  2. 2.Department of Medical BiologyCukurova University Faculty of MedicineAdanaTurkey
  3. 3.Department of Pediatric Intensive Care UnitCukurova University Faculty of MedicineAdanaTurkey
  4. 4.Institute of Health Sciences, Stem Cell DepartmentKocaeli University Center for Stem Cell and Gene Therapies Research and PracticeUmuttepe-İzmitTurkey
  5. 5.Department of PathologyCukurova University Medical FacultyAdanaTurkey
  6. 6.Department of HistologyCukurova University Faculty of MedicineAdanaTurkey
  7. 7.Department of Pediatric Hematology/OncologyCukurova University Faculty of MedicineAdanaTurkey
  8. 8.Department of Experimental Research and Practice Center of MedicalCukurova University Faculty of MedicineAdanaTurkey
  9. 9.Institute fo Health Sciences,Stem Cell DepartmentKocaeli University Center for Stem Cell and Gene Therapies research and PracticeAdanaTurkey

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