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Stem Cell Reviews and Reports

, Volume 8, Issue 3, pp 953–962 | Cite as

Stem Cell Therapy in Spinal Cord Injury: In Vivo and Postmortem Tracking of Bone Marrow Mononuclear or Mesenchymal Stem Cells

  • Mevci OzdemirEmail author
  • Ayhan Attar
  • Isinsu Kuzu
  • Murat Ayten
  • Enver Ozgencil
  • Melih Bozkurt
  • Klara Dalva
  • Duygu Uckan
  • Emine Kılıc
  • Tanzer Sancak
  • Yucel Kanpolat
  • Meral BeksacEmail author
Article

Abstract

Objective

The aim of this study was to address the question of whether bone marrow-originated mononuclear cells (MNC) or mesenchymal stem cells (MSC) induce neural regeneration when implanted intraspinally.

Materials and Methods

The study design included 4 groups of mice: Group 1, non-traumatized control group; Groups 2, 3 and 4 spinal cord traumatized mice with 1 g force Tator clips, which received intralesionally either no cellular implants (Group 2), luciferase (Luc) (+) MNC (Group 3) or MSC (Group 4) obtained from CMV-Luc or beta-actin Luc donor transgenic mice. Following the surgery until decapitation, periodical radioluminescence imaging (RLI) and Basso Mouse Scale (BMS) evaluations was performed to monitor neural activity. Postmortem immunohistochemical techniques were used to analyze the fate of donor type implanted cells.

Results

All mice of Groups 3 and 4 showed various degrees of improvement in the BMS scores, whereas there was no change in Groups 1 and 2. The functional improvement was significantly better in Group 4 compared to Group 3 (18 vs 8, p = 0.002). The immunohistochemical staining demonstrated GFP+Luc+ neuronal/glial cells that were also positive with one or more of these markers: nestin, myelin associated glycoprotein, microtubule associated protein or myelin oligodendrocyte specific protein, which is considered as indicator of donor type neuronal regeneration. Frequency of donor type neuronal cells; Luc + signals and median BMS scores were observed 48–64 % and 68–72 %; 44–80 %; 8 and 18 within Groups III and IV respectively.

Discussion

MSCs were more effective than MNC in obtaining neuronal recovery. Substantial but incomplete functional improvement was associated with donor type in vivo imaging signals more frequently than the number of neuronal cells expressing donor markers in spinal cord sections in vitro. Our results are in favor of functional recovery arising from both donor MSC and MNCs, contributing to direct neuronal regeneration and additional indirect mechanisms.

Keywords

Mesenchymal stem cell Regeneration Spinal cord Stem cell Trauma 

Notes

Acknowledgment

This study was supported by grants from the Turkish Scientific Research Council TUBİTAK, Ankara University Research Resources (SBAG) and the Turkish Academy of Sciences.

Conflicts of Interest

The authors declare no potential conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mevci Ozdemir
    • 1
    Email author
  • Ayhan Attar
    • 2
  • Isinsu Kuzu
    • 3
  • Murat Ayten
    • 4
  • Enver Ozgencil
    • 5
  • Melih Bozkurt
    • 2
  • Klara Dalva
    • 6
  • Duygu Uckan
    • 7
  • Emine Kılıc
    • 7
  • Tanzer Sancak
    • 8
  • Yucel Kanpolat
    • 9
  • Meral Beksac
    • 6
    Email author
  1. 1.School of Medicine, Department of NeurosurgeryPamukkale UniversityDenizliTurkey
  2. 2.School of Medicine, Department of NeurosurgeryAnkara UniversityAnkaraTurkey
  3. 3.School of Medicine, Department of PathologyAnkara UniversityAnkaraTurkey
  4. 4.Neurosurgery ClinicAnkara Guven HospitalAnkaraTurkey
  5. 5.School of Medicine, Department of AnesthesiologyAnkara UniversityAnkaraTurkey
  6. 6.School of Medicine, Department of HeamatologyAnkara UniversityAnkaraTurkey
  7. 7.School of Medicine, Department of Pediatric HematologyHacettepe UniversityAnkaraTurkey
  8. 8.School of Medicine, Department of RadiologyUfuk UniversityAnkaraTurkey
  9. 9.School of Medicine, Department of Neurosurgery, President, The Turkish Academi of SciencesEmeritus, Ankara UniversityAnkaraTurkey

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