Functional Characterization and Gene Expression Profiling of α-Smooth Muscle Actin Expressing Cardiomyocytes Derived from Murine Induced Pluripotent Stem Cells
- 317 Downloads
Pluripotent embryonic stem cells (ESCs) are capable of self-renewal and differentiation into specialized somatic cell types in vitro (for review see [1, 2, 3, 4]). ESCs can serve as a versatile in vitro model, including in vitro developmental biology, drug discovery and cell replacement therapies of degenerative diseases [1, 2, 3, 4]. However, sophisticated differentiation protocols and understanding of molecular and cellular mechanisms involved in differentiation processes is required to understand the physiological and functional identity of the differentiated somatic cells. This is a prerequisite for establishing robust in vitro ESC-based models for research and for regenerative medicine. In this context, we recently generated and characterized on the functional and transcriptional level several transgenic murine mesodermal ESC lineages including the α-smooth muscle (Acta2) lineage [5, 6, 7, 8, 9, 10].
During embryonic development the heart is the first organ to be...
KeywordsCaffeine Leukemia Inhibitory Factor KEGG Pathway Embryoid Body Adult Heart
Sources of Funding
This work was supported by the grant, High Yield and Performance Stem Cell Lab (Hyperlab) from the European Community, FP7 Framework Programme, Thematic Priority, Life sciences, genomics and biotechnology for health (contract 223011).
(AVI 6666 kb)
- 10.Doss, M. X., Wagh, V., Schulz, H., Kull, M., Kolde, R., Pfannkuche, K. et al. (2010). Global transcriptomic analysis of murine embryonic stem cell-derived brachyury (T) cells. Genes to Cells, 15(3), 209–228.Google Scholar
- 12.Ya, J., Markman, M. W., Wagenaar, G. T., Blommaart, P. J., Moorman, A. F., & Lamers, W. H. (1997). Expression of the smooth-muscle proteins alpha-smooth-muscle actin and calponin, and of the intermediate filament protein desmin are parameters of cardiomyocyte maturation in the prenatal rat heart. The Anatomical Record, 249(4), 495–505.PubMedCrossRefGoogle Scholar
- 19.Pfannkuche, K., Liang, H., Hannes, T., Xi, J., Fatima, A., Nguemo, F., et al. (2009). Cardiac myocytes derived from murine reprogrammed fibroblasts: intact hormonal regulation, cardiac ion channel expression and development of contractility. Cellular Physiology and Biochemistry, 24(1–2), 73–86.PubMedCrossRefGoogle Scholar
- 24.Pepper, S. D., Saunders, E. K., Edwards, L. E., Wilson, C. L., & Miller, C. J. (2007). The utility of MAS5 expression summary and detection call algorithms. Bmc Bioinformatics 8, 273.Google Scholar
- 25.Smyth, G. K. (2004). Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3, Article3.Google Scholar
- 28.Mardia, K. V., Kent, J. T., & Bibby, J. M. (1979). Multivariate analysis. London: Academic.Google Scholar
- 30.Dennis, G., Sherman, B. T., Hosack, D. A., Yang, J., Gao, W., Lane, H. C. et al. (2003). DAVID: Database for annotation, visualization, and integrated discovery. Genome Biology 4(5), P3.Google Scholar
- 32.Germanguz, I., Sedan, O., Zeevi-Levin, N., Shtreichman, R., Barak, E., Ziskind, A. et al. (2011). Molecular characterization and functional properties of cardiomyocytes derived from human inducible pluripotent stem cells. Journal of Cellular and Molecular Medicine, 15(1), 38–51.Google Scholar
- 33.Chase, A., Orchard, C. H. (2011). Ca efflux via the sarcolemmal Ca ATPase occurs only in the t-tubules of rat ventricular myocytes. Journal of Molecular and Cellular Cardiology, 50(1), 187–193.Google Scholar
- 35.Banerjee, I., Fuseler, J. W., Price, R. L., Borg, T. K., & Baudino, T. A. (2007). Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse. American Journal of Physiology. Heart and Circulatory Physiology, 293(3), H1883–H1891.PubMedCrossRefGoogle Scholar