Stem Cell Reviews and Reports

, Volume 7, Issue 4, pp 898–909

Specific Unsaturated Fatty Acids Enforce the Transdifferentiation of Human Cancer Cells toward Adipocyte-like Cells

  • Antonio Ruiz-Vela
  • Cristóbal Aguilar-Gallardo
  • Ana M. Martínez-Arroyo
  • Mario Soriano-Navarro
  • Verónica Ruiz
  • Carlos Simón
Article

Abstract

Differentiation therapy pursues the discovery of novel molecules to transform cancer progression into less aggressive phenotypes by mechanisms involving enforced cell transdifferentiation. In this study, we examined the identification of transdifferentiating adipogenic programs in human cancer cell lines (HCCLs). Our findings showed that specific unsatturated fatty acids, such as palmitoleic, oleic and linoleic acids, trigger remarkable phenotypic modifications in a large number of human cancer cell lines (HCCLs), including hepatocarcinoma HUH-7, ovarian carcinoma SK-OV-3, breast adenocarcinoma MCF-7 and melanoma MALME-3M. In particular, we characterized a massive biogenesis of lipid droplets (LDs) and up-regulation of the adipogenic master regulator, PPARG, resulting in the transdifferentiation of HCCLs into adipocyte-like cells. These findings suggest the possibility of a novel strategy in cancer differentiation therapy via switching the identity of HCCLs to an adipogenic phenotype through unsaturated fatty acid-induced transdifferentiation.

Keywords

Transdifferentiation Adipocytes Lipid Droplets PPARG Unsaturated Fatty Acids 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Antonio Ruiz-Vela
    • 1
  • Cristóbal Aguilar-Gallardo
    • 1
  • Ana M. Martínez-Arroyo
    • 1
  • Mario Soriano-Navarro
    • 1
  • Verónica Ruiz
    • 1
  • Carlos Simón
    • 1
  1. 1.Valencia Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF)ValenciaSpain

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