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Stem Cell Reviews and Reports

, Volume 7, Issue 4, pp 775–781 | Cite as

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

  • Ana Maria Fraga
  • Érica Sara Souza de Araújo
  • Raquel Stabellini
  • Naja Vergani
  • Lygia V. PereiraEmail author
Article

Abstract

Since the derivation of the first human embryonic stem cell (hESC) lines by Thomson and coworkers in 1998, more than 1,200 different hESC lines have been established worldwide. Nevertheless, there is still a recognized interest in the establishment of new lines of hESC, particularly from HLA types and ethnic groups currently underrepresented among the available lines. The methodology of hESC derivation has evolved significantly since 1998, when human LIF (hLIF) was used for maintenance of pluripotency. However, there are a number of different strategies for the several steps involved in establishing a new line of hESC. Here we make a survey of the most relevant parameters used between 1998 and 2010 for the derivation of the 375 hESC lines deposited in two international stem cell registries, and able to form teratomas in immunocompromised mice. Although we identify some trends in the methodology for establishing hESC lines, our data reveal a much greater heterogeneity of strategies than what is used for derivation of murine ESC lines, indicating that optimum conditions have not been consolidated yet, and thus, hESC establishment is still an evolving field of research.

Keywords

Human embryonic stem cells Cell line derivation Pluripotency Human embryo Cell therapy 

Notes

Acknowledgements

This work was supported by a grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico/Departamento de Ciência e Tecnologia do Ministério da Saúde (CNPq/MS/DECIT). AMF, RS and ESSA have/had Ph.D. fellowships from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). The authors declare no conflicts of interest.

Supplementary material

12015_2011_9250_MOESM1_ESM.xls (178 kb)
Supplementary Table 1 Original data on the derivation of hESC lines (XLS 178 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ana Maria Fraga
    • 1
  • Érica Sara Souza de Araújo
    • 1
  • Raquel Stabellini
    • 1
    • 2
  • Naja Vergani
    • 1
  • Lygia V. Pereira
    • 1
    Email author
  1. 1.Laboratório Nacional de Células-Tronco Embrionárias and Instituto Nacional de Ciência e Tecnologia em Células-Tronco e Terapia Celular, Departamento de Genética e Biologia Evolutiva, Instituto de BiociênciasUniversidade de São PauloSão PauloBrazil
  2. 2.Programa de Transtorno Bipolar (PROMAN), Instituto de PsiquiatriaUniversidade de São PauloSão PauloBrazil

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